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Updated: Nov 4, 2025
Rapid Molecular Detection and Differentiation of Influenza Viruses A and B
Published on: January 30, 2017
[Internal validity of a rapid test for COVID-19 antigens in a nursing home]
C Diez Flecha1, A M Rivero Rodríguez1, T Fernández-Villa2
1Gerencia de Atención Primaria de León, León, España.
Introduction:
RT-qPCR is the reference test for the detection of SARS-CoV-2 infection, however, rapid antigen detection tests (RADT) are now available. In this work, the internal validity of the RADT was evaluated in the context of an outbreak in a nursing home.
Methods:
Nasopharyngeal exudate samples were analyzed by RADT and RT-qPCR from 61 residents of a nursing home. The sensitivity and specificity of RADT with respect to RT-qPCR was calculated.
Results:
Specificity was 100% (95% CI 54.1-100.0), while sensitivity in asymptomatic people was 70.3% (95% CI 53.0-84.1) and in symptomatic people 83.3% (95% CI 51.6-97.9).
Conclusions:
The RADTs are sufficiently sensitive and specific to be used as screening tests in nursing homes, especially in situations of outbreaks or suspected outbreaks due to the presence of symptoms.
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