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Rapid Verification of Terminators Using the pGR-Blue Plasmid and Golden Gate Assembly
Published on: April 25, 2016
Replication termination for staphylococcal plasmids: plasmids pT181 and pC221 cross-react in the termination process
1Department of Plasmid Biology, Public Health Research Institute, New York, New York 10016.
Abstract:
We present data which indicate that (i) the origin of replication of plasmids pT181 and pC221 can also function as termination signals; (ii) termination of replication occurs when a round of replication initiated either by RepC at the pT181 origin or by RepD at the pC221 origin reaches either of these origins, proving that the two plasmids cross-react for termination of replication; and (iii) the replication initiated at the origin of another staphylococcal plasmid, pE194, does not terminate at the origin of pT181 or pC221, indicating the existence of a specific relationship between the initiation and termination of a replication event.
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