Practical guidance for clinical laboratories for SARS-CoV-2 serology testing

Area of Science:

• Virology
• Immunology
• Public Health

Background:

• The landscape of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) diagnostic testing is rapidly evolving.
• Serology testing, while limited for acute infection diagnosis, is increasingly valuable for population-level insights and public health decision-making.
• The global rollout of vaccines necessitates antibody screening and interpretation for both vaccinated and unvaccinated individuals.

Purpose of the Study:

• To present updated data on SARS-CoV-2 antibody timelines, including antibody longevity and neutralizing antibody production.
• To offer practical guidance for clinical microbiology laboratories on verifying commercial serology assays.
• To assist laboratories in selecting appropriate testing algorithms for their specific populations.

Main Methods:

• Review of current data on SARS-CoV-2 antibody kinetics.
• Analysis of neutralizing antibody production and detection.
• Guidance on assay verification and algorithm selection for clinical laboratories.

Main Results:

• Provides the most up-to-date information on SARS-CoV-2 antibody timelines and longevity.
• Details the production and detection of neutralizing antibodies.
• Offers practical recommendations for clinical laboratory practices.

Conclusions:

• Serology plays a vital role in assessing population immunity and informing public health strategies.
• Clinical laboratories require updated guidance for antibody testing and interpretation in the context of vaccination.
• Effective laboratory strategies are essential for managing SARS-CoV-2 diagnostics.