Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Interactions between ascorbic acid and linear and cyclized bile pigments. A thin-layer chromatographic study.

M Barbier1

  • 1Institut de Chimie des Substances Naturelles, CNRS, Gif-sur-Yvette, France.

Journal of Chromatography
|May 25, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defining the role of <i>Pseudomonas aeruginosa</i> PilY1 in signaling and virulence.

Journal of bacteriology·2026
Same author

Plastic Type and Condition Have Minimal Impact on Associated Marine Biofilm Communities.

Environmental microbiology·2025
Same author

Microreversibility and the statistics of currents in quantum transport.

Physical review. E·2020
Same author

[How I explore … abdominal tuberculosis in children ?]

Revue medicale de Liege·2020
Same author

A cost-effectiveness analysis of consolidation immunotherapy with durvalumab in stage III NSCLC responding to definitive radiochemotherapy in Switzerland.

Annals of oncology : official journal of the European Society for Medical Oncology·2020
Same author

[Focus on the endocannabinoid system and the reprotoxicity of marijuana in female users].

Gynecologie, obstetrique, fertilite & senologie·2020

Ascorbic acid interacts with bile pigments, with reactivity increasing with molecular cyclization. This study quanties the interaction between ascorbic acid and sarpedobilin, revealing its potential biochemical significance.

Area of Science:

  • Biochemistry
  • Organic Chemistry

Background:

  • Bile pigments, such as biliverdin derivatives, are products of heme degradation.
  • Understanding their interactions with other molecules is crucial for elucidating their biochemical roles.

Purpose of the Study:

  • To investigate the physico-chemical interactions between ascorbic acid and linear or cyclized bile pigment dimethyl esters.
  • To establish the relationship between bile pigment cyclization and reactivity with ascorbic acid.

Main Methods:

  • Thin-layer chromatography on silica gel using an interacting barrier technique.
  • Spectrophotometric analysis of the ascorbic acid-sarpedobilin interaction.

Main Results:

  • Reactivity of bile pigments with ascorbic acid increases with the degree of molecular cyclization.

Related Experiment Videos

  • Linear biliverdin-type pigments showed low reactivity, while monocyclic pigments exhibited medium to strong interactions.
  • The natural pigment sarpedobilin was strongly captured by ascorbic acid.
  • Spectrophotometry indicated that over 2 moles of ascorbic acid are required per mole of sarpedobilin.

    • Conclusions:

    • The degree of cyclization significantly influences the interaction strength between bile pigments and ascorbic acid.
    • The findings suggest a potential biochemical role for these interactions, particularly involving sarpedobilin and ascorbic acid.