Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 10, 2026

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

Chromogranin A expression in normal and malignant human tissues.

L J Helman1, A F Gazdar, J G Park

  • 1Naval Medical Oncology Branch, National Cancer Institute, Bethesda, Maryland 20892.

The Journal of Clinical Investigation
|August 1, 1988
PubMed
Summary

Chromogranin A mRNA expression, typically neuroendocrine, was found in some colon adenocarcinomas. This discovery suggests a new tumor subgroup with neuroendocrine features, aiding cancer classification.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

EYA4 is inactivated biallelically at a high frequency in sporadic lung cancer and is associated with familial lung cancer risk.

Oncogene·2013
Same author

Oncogenic KRAS-induced epiregulin overexpression contributes to aggressive phenotype and is a promising therapeutic target in non-small-cell lung cancer.

Oncogene·2012
Same author

Enteropathy-associated T-cell lymphoma in a patient without a prior diagnosis of celiac disease: a diagnostic conundrum.

Indian journal of cancer·2011
Same author

Should we continue to use the term non-small-cell lung cancer?

Annals of oncology : official journal of the European Society for Medical Oncology·2010
Same author

DNA methylation based biomarkers in non-invasive cancer screening.

Current molecular medicine·2010
Same author

Epidermal growth factor receptor regulates MET levels and invasiveness through hypoxia-inducible factor-1alpha in non-small cell lung cancer cells.

Oncogene·2010

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Chromogranin A (CHGA) is a protein widely expressed in neuroendocrine tissues.
  • Its mRNA expression serves as a marker for neuroendocrine differentiation.

Purpose of the Study:

  • To investigate the expression of chromogranin A mRNA in various human tissues and tumors.
  • To determine if CHGA expression is present in non-neuroendocrine tumors like colon adenocarcinoma.

Main Methods:

  • Northern blot analysis to detect CHGA mRNA.
  • In situ hybridization to localize CHGA mRNA expression within tissues.

Main Results:

  • CHGA mRNA was predominantly found in normal tissues and tumors of neuroendocrine origin.

More Related Videos

Identifying, Diagnosing, and Grading Malignant Peripheral Nerve Sheath Tumors in Genetically Engineered Mouse Models
08:57

Identifying, Diagnosing, and Grading Malignant Peripheral Nerve Sheath Tumors in Genetically Engineered Mouse Models

Published on: May 17, 2024

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker
07:47

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker

Published on: September 15, 2023

Related Experiment Videos

Last Updated: Jul 10, 2026

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections
05:45

Quantitative Immunohistochemistry of the Cellular Microenvironment in Patient Glioblastoma Resections

Published on: July 31, 2017

Identifying, Diagnosing, and Grading Malignant Peripheral Nerve Sheath Tumors in Genetically Engineered Mouse Models
08:57

Identifying, Diagnosing, and Grading Malignant Peripheral Nerve Sheath Tumors in Genetically Engineered Mouse Models

Published on: May 17, 2024

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker
07:47

Multiomics Analysis of TMEM200A as a Pan-Cancer Biomarker

Published on: September 15, 2023

  • Notably, CHGA mRNA was detected in 2 out of 13 colon adenocarcinoma specimens.
  • This indicates a potential neuroendocrine component in a subset of colon cancers.

    • Conclusions:

    • The presence of CHGA mRNA in colon cancer suggests a previously unrecognized neuroendocrine subgroup.
    • This finding highlights the utility of molecular techniques for refining tumor classification.
    • Recombinant DNA technology aids in identifying novel tumor subtypes and improving diagnostic accuracy.