Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

58.2K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
58.2K
Alternative RNA Splicing02:18

Alternative RNA Splicing

23.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
23.0K
Alternative RNA Splicing02:18

Alternative RNA Splicing

4.3K
4.3K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

2.8K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
2.8K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.6K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.6K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.0K
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sphingosine-1-phosphate receptor 1 signaling stimulates human pluripotent stem cell-derived cardiomyocyte differentiation and maturation.

Experimental & molecular medicine·2026
Same author

Development of a LC-MS-based simultaneous protein quantification method for all regulated allergenic foods in Korea.

Food chemistry·2026
Same author

Secretory LGALS3BP exacerbates sepsis-associated liver dysfunction by activating inflammasome-mediated pyroptosis.

Cell death discovery·2026
Same author

A Giant Exophytic Gastric GIST Mimicking Ovarian Cancer: A Diagnostic Pitfall on CT and [<sup>18</sup>F]FDG PET/CT.

Diagnostics (Basel, Switzerland)·2026
Same author

LGALS3BP Links Centrosomes and Mitochondria to Maintain Energetic Fitness and Restrain Compensatory Lipid Catabolic Reprogramming in Hepatocellular Carcinoma.

Cancer genomics & proteomics·2026
Same author

Design and rationale of the clinical trial to obtain the highest efficacy of dual antiplatelet therapy after carotid artery stenting in high bleeding risk patients (CHET): A multicenter, randomized, open-label, superiority trial.

American heart journal·2026

Related Experiment Video

Updated: Nov 4, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.0K

NSrp70 is a lymphocyte-essential splicing factor that controls thymocyte development.

Chang-Hyun Kim1,2, Sang-Moo Park1,2, Sun-Jae Lee1

  • 1School of Life Sciences, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Korea.

Nucleic Acids Research
|May 26, 2021
PubMed
Summary

NSrp70, a splicing factor, is vital for T cell development in the thymus. Its absence causes severe immune defects and impacts tumor control, highlighting its role in T cell maturation.

More Related Videos

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.2K
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.7K

Related Experiment Videos

Last Updated: Nov 4, 2025

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

3.0K
Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.2K
ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast
07:31

ACT1-CUP1 Assays Determine the Substrate-Specific Sensitivities of Spliceosomal Mutants in Budding Yeast

Published on: June 30, 2022

2.7K

Area of Science:

  • Molecular Biology
  • Immunology
  • Developmental Biology

Background:

  • Alternative pre-mRNA splicing expands proteomic diversity.
  • Lineage-specific splicing regulators in physiology are poorly understood.
  • NSrp70 is selectively expressed in developing thymocytes, peaking at the double-positive (DP) stage.

Purpose of the Study:

  • Investigate the function of NSrp70 in thymocyte development.
  • Determine the role of NSrp70 in regulating cell cycle and survival.
  • Elucidate the impact of NSrp70 deficiency on T cell maturation and immune function.

Main Methods:

  • Global splicing and transcriptional profiling.
  • Conditional knockout of Nsrp1 (NSrp70-cKO) using CD4Cre.
  • Analysis of T cell maturation, TCR signaling, cell growth, and death.

Main Results:

  • NSrp70 controls thymocyte cell cycle and survival by regulating splicing factors, including SRSF1.
  • NSrp70-cKO mice exhibit defects in T cell maturation to single-positive thymocytes.
  • Defects include insufficient TCR signaling, uncontrolled cell growth/death, peripheral lymphopenia, and impaired tumor control.

Conclusions:

  • NSrp70 is a critical lymphoid-lineage-specific splicing factor in thymic T cell development.
  • NSrp70 regulates key pathways governing T cell maturation, survival, and immune surveillance.
  • This study provides a model for understanding lineage-specific splicing factor function in immunity.