Related Experiment Video

Updated: Nov 4, 2025

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells
07:23

Dual CRISPR-Interference Strategy for Targeting Synthetic Lethal Interactions Between Non-Coding RNAs in Cancer Cells

Published on: May 30, 2025

801

Long Noncoding RNA LINC01410 Suppresses Tumorigenesis and Enhances Radiosensitivity in Neuroblastoma Cells Through

Liping Mou1, Lili Wang2, Shaoming Zhang3

  • 1Department of Child Healthcare, People's Hospital of Rizhao, Rizhao, 276800, Shandong, People's Republic of China.

Oncotargets and Therapy
|May 27, 2021
PubMed
Abstract

Insights

Long noncoding RNA LINC01410 promotes neuroblastoma (NB) by increasing proliferation and decreasing radiosensitivity. Inhibiting LINC01410, via the miR-545-3p/HK2 pathway, offers a potential therapeutic strategy for NB treatment.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Aberrant expression of long noncoding RNAs (lncRNAs) is implicated in cancer development and response to radiation therapy.
  • Neuroblastoma (NB) is a pediatric cancer where lncRNA dysregulation may play a role in tumorigenesis and radiosensitivity.

Purpose of the Study:

  • To investigate the function and regulatory mechanism of lncRNA LINC01410 in neuroblastoma.
  • To determine the role of LINC01410 in NB tumorigenesis and its sensitivity to radiation.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) for gene expression analysis.
  • Cell viability, colony formation, invasion, glucose consumption, and lactate production assays to assess tumorigenesis and radiosensitivity.
  • Dual-luciferase reporter, RNA Immunoprecipitation (RIP), and RNA pull-down assays to confirm molecular interactions.
  • Western blot and in vivo mice xenograft models to validate findings.

Main Results:

  • LINC01410 and HK2 were upregulated, while miR-545-3p was downregulated in NB tissues and cells.
  • LINC01410 knockdown suppressed NB cell proliferation, invasion, and tumor growth in vivo, while enhancing radiosensitivity by inhibiting glycolysis.
  • LINC01410 acts as a molecular sponge for miR-545-3p, regulating the expression of HK2, a target of miR-545-3p.

Conclusions:

  • LINC01410 interference inhibits neuroblastoma tumorigenesis and enhances radiosensitivity.
  • The LINC01410/miR-545-3p/HK2 axis represents a novel therapeutic target for neuroblastoma.
Keywords:
HK2LINC01410NBmiR-545-3pradiosensitivitytumorigenesis

More Related Videos

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.6K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.2K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.1K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.7K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
5.4K