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Molecular cloning and sequence analysis of the mumps virus gene encoding the P protein: mumps virus P gene is
K Takeuchi1, M Hishiyama, A Yamada
1Department of Measles Virus, National Institute of Health, Tokyo, Japan.
Abstract:
The nucleotide sequence of the P (phosphoprotein) gene of two strains of mumps virus has been determined from overlapping cDNA clones. The P gene contained a single open reading frame coding for a protein of 391 amino acids with a calculated Mr of 41,587, in good agreement with the value (40K to 45K) estimated from electrophoretic mobility on SDS-polyacrylamide gels. No open reading frame analogous to the C gene of other paramyxoviruses existed in the mumps virus P gene region. Comparison of the amino acid sequence of the mumps virus P protein with that of Newcastle disease virus showed a limited sequence homology.
Insights
The mumps virus phosphoprotein (P) gene sequence was determined, revealing a single open reading frame. This P protein shows limited homology to Newcastle disease virus, aiding in understanding paramyxovirus evolution.
Area of Science:
- Virology
- Molecular Biology
- Genetics
Background:
- Mumps virus is a significant human pathogen belonging to the Paramyxoviridae family.
- The phosphoprotein (P) gene plays a crucial role in viral replication and transcription.
Purpose of the Study:
- To determine the nucleotide sequence of the mumps virus P gene.
- To analyze the predicted amino acid sequence and compare it with other paramyxoviruses.
Main Methods:
- Nucleotide sequencing of cDNA clones derived from mumps virus RNA.
- Bioinformatic analysis to identify open reading frames and predict protein characteristics.
- Amino acid sequence comparison using computational methods.
Main Results:
- The mumps virus P gene sequence was elucidated, encoding a protein of 391 amino acids.
- The calculated molecular weight of the P protein (41,587 Da) aligns with experimental estimates.
- No C gene homolog was found in the mumps virus P gene region, unlike other paramyxoviruses.
- Limited amino acid sequence homology was observed between mumps virus P protein and Newcastle disease virus P protein.
Conclusions:
- The determined mumps virus P gene sequence provides a foundational resource for further molecular studies.
- The absence of a C gene homolog suggests potential differences in the replication strategy of mumps virus compared to other paramyxoviruses.
- The observed limited homology highlights the evolutionary divergence within the Paramyxoviridae family.