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Published on: March 10, 2020
Blastocystis Subtypes and Culture Characteristics of Isolates from Human Stools Related with the Presence of
Deicy Muñoz-Sánchez1, Jessica Triviño-Valencia1, Fabiana Lora-Suarez1
1Molecular Parasitology Group (GEPAMOL, for the Term in Spanish), Biomedical Research Center, Faculty of Health Sciences, Universidad del Quindío, Armenia, Colombia.
Purpose:
To stablish if Blastocystis subtypes influences gastrointestinal symptoms.
Methods:
Case-control study. We obtained sequencing for Blastocysts subtyping from 13 patients with gastrointestinal symptoms (diarrhea or abdominal pain) and 12 from individuals without symptoms.
Results:
12 sequences were from Subtype 2 and one from Subtype 3 in symptomatic individuals and nine samples were from Subtype 1, one from Subtype 2, and two from Subtype 3 in asymptomatic individuals. The prevalence of subtype 2 in symptomatic individuals was vastly different compared to the frequency in asymptomatic individuals (84.6% vs. 16.6%; OR 27.5 95% CI 3.2-233; Fisher exact test p = 0.0010201335). After in vitro culture, 22 isolates were obtained. Significant differences were observed for the 12 isolates from Subtype 2 that get a smaller number of total cells with dominant growth of vacuolar forms, compared with Subtypes 1 and 3, after eight days of culture.
Conclusion:
Our results suggest that gastrointestinal symptoms in Colombian individuals with Blastocystis infection depend on the infecting subtype with peculiar phenotypic characteristics in in vitro culture.
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