Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spectrophotometry: Introduction01:16

Spectrophotometry: Introduction

5.7K
Spectrophotometry is the quantitative measurement of the absorption, reflection, diffraction, or transmission of electromagnetic radiation through a material as a function of the intensity and wavelength of the radiation. A spectrophotometer is a device used to measure the change in the radiation intensity caused by its interaction with the material.
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
5.7K
Applications of IR Spectroscopy: Overview01:11

Applications of IR Spectroscopy: Overview

1.6K
The non-destructive nature and ability to provide valuable chemical information make IR spectroscopy a versatile technique with broad applications in various scientific and industrial fields. IR spectroscopy is commonly used to identify and characterize organic and inorganic compounds. It provides information about the functional groups present in a molecule and the bonding between atoms. This helps in the structural elucidation of compounds during organic synthesis, pharmaceutical research,...
1.6K
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview01:02

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

3.8K
Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material,  molecules absorb light depending on the energy required for...
3.8K
Raman Spectroscopy: Overview01:20

Raman Spectroscopy: Overview

855
The underlying principle of Raman spectroscopy is based on the interaction between light and matter, specifically molecules' inelastic scattering of photons. When a monochromatic beam of light, typically from a laser source, interacts with a sample, most scattered light has the same frequency as the incident light. This is known as Rayleigh scattering.
However, a small fraction of the scattered light exhibits a frequency shift due to the exchange of energy between the incident photons and...
855

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Combined multi-omics and multi-spectral profiling of plasma extracellular vesicles reveals liquid biopsy biomarkers for glioma diagnosis.

Cell reports. Medicine·2026
Same author

A Multiomic Liquid Biopsy for the Earlier Detection of Colorectal Cancer.

Cancer prevention research (Philadelphia, Pa.)·2025
Same author

From Lab to Clinic: Artificial Intelligence with Spectroscopic Liquid Biopsies.

Diagnostics (Basel, Switzerland)·2025
Same author

Colorectal cancer molecular profiling: Opportunities for early detection.

Clinical and translational medicine·2025
Same author

Machine-learning based classification of 2D-IR liquid biopsies enables stratification of melanoma relapse risk.

Chemical science·2025
Same author

Bioprocess monitoring applications of an innovative ATR-FTIR spectroscopy platform.

Frontiers in bioengineering and biotechnology·2024

Related Experiment Video

Updated: Nov 4, 2025

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

8.0K

Clinical Spectroscopy: Lost in Translation?

James M Cameron1, Christopher Rinaldi2, Samantha H Rutherford2

  • 1ClinSpec Diagnostics Ltd, Glasgow, UK.

Applied Spectroscopy
|May 27, 2021
PubMed
Summary

Biomedical Raman and infrared spectroscopy show promise for clinical use, but face technical, economic, and regulatory barriers. Overcoming these challenges is crucial for translating spectroscopic technologies into effective medical diagnostics and applications.

Keywords:
FT-IRFourier transform infraredInfraredRamanbiomedicalclinical translationdiagnosticsvibrational spectroscopy

More Related Videos

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
07:11

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

Published on: August 19, 2021

2.7K
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.5K

Related Experiment Videos

Last Updated: Nov 4, 2025

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis
10:35

Multimodal Imaging and Spectroscopy Fiber-bundle Microendoscopy Platform for Non-invasive, In Vivo Tissue Analysis

Published on: October 17, 2016

8.0K
ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis
07:11

ARL Spectral Fitting as an Application to Augment Spectral Data via Franck-Condon Lineshape Analysis and Color Analysis

Published on: August 19, 2021

2.7K
High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology
11:05

High-definition Fourier Transform Infrared FT-IR Spectroscopic Imaging of Human Tissue Sections towards Improving Pathology

Published on: January 21, 2015

33.5K

Area of Science:

  • Biomedical engineering
  • Spectroscopy
  • Medical diagnostics

Background:

  • Vibrational spectroscopy, including Raman and infrared, has shown significant potential in biomedical science.
  • Advancements in computational methods have supported numerous proof-of-concept studies for spectroscopic approaches.

Purpose of the Study:

  • To review the development of biomedical Raman and infrared spectroscopy.
  • To identify and discuss the barriers hindering the clinical translation of these technologies.

Main Methods:

  • Literature review of studies published over the past few decades.
  • Analysis of technical, clinical, and economic challenges to adoption.

Main Results:

  • Numerous proof-of-concept studies highlight the potential of spectroscopic methods.
  • Significant hurdles remain, including study limitations, lack of clinical pathway understanding, regulatory approval, and funding.

Conclusions:

  • Biomedical vibrational spectroscopy must address identified barriers to achieve clinical efficacy.
  • Future directions are advised to facilitate the integration of spectroscopic technologies into medical diagnostics and clinical practice.