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Related Concept Videos

Proteomics01:33

Proteomics

8.7K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
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The Tumor Microenvironment02:17

The Tumor Microenvironment

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Every normal cell or tissue is embedded in a complex local environment called stroma, consisting of different cell types, a basal membrane, and blood vessels. As normal cells mutate and develop into cancer cells, their local environment also changes to allow cancer progression. The tumor microenvironment (TME) consists of a complex cellular matrix of stromal cells and the developing tumor. The cross-talk between cancer cells and surrounding stromal cells is critical to disrupt normal tissue...
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Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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Spatial proteomics for understanding the tissue microenvironment.

Yiheng Mao1, Xi Wang2, Peiwu Huang3

  • 1School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin, 150001, China. tianrj@sustech.edu.cn and Department of Chemistry, College of Science, Southern University of Science and Technology, Shenzhen 518055, China.

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|May 27, 2021
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Summary
This summary is machine-generated.

Spatial proteomics advances our understanding of the tissue microenvironment, particularly the tumor microenvironment (TME). This review covers mass spectrometry-based spatial proteomics achievements, challenges, and future directions.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Proteomics

Background:

  • The human body consists of complex cellular ecosystems within tissue microenvironments.
  • Understanding these microenvironments, especially the tumor microenvironment (TME), is crucial for disease research.
  • Spatial proteomics aims to map protein distribution within these tissues.

Purpose of the Study:

  • To review the achievements and challenges in mass spectrometry-based tissue spatial proteomics.
  • To highlight technological advancements and biomedical applications in spatial proteomics.
  • To discuss future directions for the field.

Main Methods:

  • Scalpel macrodissection-based region-resolved proteomics for high-quality resource generation.
  • Laser microdissection-based spatial proteomics with sensitive sample preparation.
  • Antibody recognition-based multiplexed tissue imaging techniques.

Main Results:

  • Significant progress has been made in characterizing protein abundance and spatial distribution in diseased tissues.
  • Key technologies like macrodissection and microdissection-based proteomics have been developed.
  • Multiplexed imaging techniques offer new avenues for spatial protein analysis.

Conclusions:

  • Mass spectrometry-based spatial proteomics is a rapidly evolving field with major implications for biomedical research.
  • Continued technological development is essential to overcome current challenges.
  • Future research should focus on refining methods and expanding applications, particularly in TME studies.