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Updated: Nov 4, 2025

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Published on: February 11, 2022

Infrared Based Saliva Screening Test for COVID-19.

Bayden R Wood^1,2, Kamila Kochan¹, Diana E Bedolla^1,3,4

¹Centre for Biospectroscopy and School of Chemistry, Monash University, Clayton Campus, 3800, Victoria, Australia.

Angewandte Chemie (International Ed. in English)

|May 27, 2021

Summary

A new portable infrared spectrometer offers rapid, point-of-care detection of COVID-19 markers in saliva. This inexpensive, high-throughput test shows 93% sensitivity and 82% specificity, ideal for mass screening.

Keywords:

COVID-19 diagnostic Fourier transform infrared (FTIR) spectroscopy Raman spectroscopy SARS-CoV-2 saliva

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Area of Science:

Spectroscopy
Biomedical Engineering
Infectious Disease Diagnostics

Background:

The COVID-19 pandemic necessitates rapid and accessible diagnostic testing for effective control.
Current diagnostic methods can be time-consuming or require specialized laboratory facilities.

Purpose of the Study:

To develop and validate a portable infrared spectrometer system for rapid, point-of-care COVID-19 detection.
To assess the sensitivity and specificity of the infrared spectroscopy method for identifying SARS-CoV-2 markers in saliva.

Main Methods:

Characterization of SARS-CoV-2 virions using Raman spectroscopy, synchrotron infrared (IR), and AFM-IR.
Acquisition of 171 transflection infrared spectra from saliva samples of 29 SARS-CoV-2 positive and 28 negative individuals.
Analysis of spectral data using Monte Carlo Double Cross Validation.

Main Results:

The infrared spectroscopy test achieved a sensitivity of 93% (27/29) and a specificity of 82% (23/28).
The method demonstrated effectiveness even for samples near the limit of detection for RT-qPCR.
The system is designed for high throughput, rapid results, and utilizes self-collected saliva samples.

Conclusions:

A proof-of-concept for a high-throughput, rapid, and portable infrared COVID-19 test has been demonstrated.
The developed system minimizes risk to healthcare workers through sample self-collection.
This technology is well-suited for mass screening efforts to control the spread of COVID-19.