Vesicle traffic in the outer hair cell.
Csaba Harasztosi1, Entcho Klenske1, Anthony W Gummer1
1Section of Physiological Acoustics and Communication, Faculty of Medicine, Eberhard Karls University Tübingen, Tübingen, Germany.
The European Journal of Neuroscience
|May 27, 2021
Summary
Outer hair cells use both clathrin-dependent and independent endocytosis. Myosin VI transports vesicles bidirectionally, while kinesin and dynein facilitate basoapical trafficking, crucial for hearing.
Area of Science:
- Cell Biology
- Auditory Neuroscience
Background:
- Outer hair cells (OHCs) are vital for hearing, relying on precise protein recycling.
- Understanding endocytic and transcytic mechanisms in OHCs is key to auditory perception.
Purpose of the Study:
- To investigate the roles of different endocytic pathways in OHCs.
- To elucidate the mechanisms of vesicle trafficking along and across OHCs.
Main Methods:
- Utilized the plasma-membrane marker FM1-43 in isolated guinea-pig cochlear outer hair cells.
- Employed a double-barrel local perfusion system to target apical or synaptic poles.
- Applied inhibitors for macropinocytosis/phagocytosis (phenylarsine oxide), clathrin-mediated endocytosis (concanavalin A), myosin VI (2,4,6-triiodophenol), kinesin (monastrol), and dynein (ciliobrevin D).
Main Results:
- Both clathrin-dependent and independent endocytosis occur at apical and synaptic poles.
- Clathrin-independent processes are more significant for basal pole endocytosis.
- Myosin VI mediates bidirectional vesicle transport and radial movement to subsurface cisternae.
- Kinesin and dynein are primarily involved in basoapical vesicle trafficking.
Conclusions:
- OHCs employ diverse endocytic mechanisms at both poles.
- Myosin VI, kinesin, and dynein play distinct roles in OHC vesicle trafficking.
- This research provides fundamental insights into OHC protein recycling and auditory function.
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