Sensitive identification of silibinin as anticancer drug in human plasma samples using poly (β-CD)-AgNPs: A new...

Area of Science:

• Biomedical analysis
• Electrochemistry
• Nanotechnology

Background:

• Silibinin exhibits significant anti-neoplastic effects against various cancer types.
• Accurate and sensitive detection of silibinin in clinical samples is crucial for cancer therapy.
• Existing methods for silibinin analysis may lack the required sensitivity or speed for clinical applications.

Purpose of the Study:

• To develop and validate an innovative electroanalysis method for silibinin detection in human plasma.
• To create a sensitive and rapid sensing platform for biomedical analysis of silibinin.
• To establish a reliable method for monitoring silibinin concentration in real clinical samples.

Main Methods:

• Fabrication of a sensing platform using silver nanoparticles (AgNPs) on a β-cyclodextrin (β-CD) polymeric layer.
• Utilizing cubic AgNPs to enhance surface area for β-CD electropolymerization, improving electron conductivity.
• Employing differential pulse voltammetry for quantitative analysis of silibinin in human plasma samples.

Main Results:

• The sensor demonstrated high electron conductivity and efficient interaction with silibinin.
• A linear relationship was observed between silibinin concentration (0.0103–10.3 µM) and the anodic peak current.
• The method was validated for accurate and sensitive detection in both standard and human plasma samples.

Conclusions:

• The proposed electroanalysis method offers an accurate, sensitive, and rapid approach for silibinin recognition in human plasma.
• The developed sensor platform is efficient for monitoring silibinin in clinical settings.
• This innovation supports the effective therapy of cancer through precise silibinin level assessment.