Related Experiment Video
Updated: Nov 4, 2025

Swabbing the Urban Environment - A Pipeline for Sampling and Detection of SARS-CoV-2 From Environmental Reservoirs
Published on: April 9, 2021
SARS-CoV-2 aerosol generation during respiratory equipment reprocessing
Camila Quartim de Moraes Bruna1,2, Caroline Lopes Ciofi-Silva3, Anderson Vicente de Paula4
1Escola de Enfermagem da Universidade de São Paulo, Av Dr Enéas de Carvalho Aguiar, 419, Cerqueira Cesar, São Paulo, SP, 05403-000, Brazil. caquartim@usp.br.
Reprocessing medical devices contaminated with coronavirus disease 2019 (COVID-19) does not appear to aerosolize SARS-CoV-2. Standard medical masks are likely sufficient protection for sterile processing departments during the pandemic.
Area of Science:
- Infection Control
- Public Health
- Healthcare Associated Infections
Background:
- The COVID-19 pandemic raised concerns about potential SARS-CoV-2 aerosolization during medical device reprocessing.
- Understanding airborne transmission risks is crucial for protecting healthcare workers in sterile processing departments.
Purpose of the Study:
- To assess the presence of SARS-CoV-2 in the air and on surfaces within sterile processing departments during device reprocessing.
- To determine if reprocessing contaminated respiratory equipment leads to airborne viral shedding.
Main Methods:
- Air and surface samples were collected from sterile processing departments and ICUs in two hospitals.
- Samples were taken during the reprocessing of respiratory equipment from COVID-19 patients.
- RT-PCR was used to detect SARS-CoV-2 RNA.
Main Results:
- SARS-CoV-2 was detected in only one air sample prior to decontamination.
- No viable SARS-CoV-2 RNA was found in any sterile processing department or patient-adjacent samples.
- Reprocessing of respiratory equipment did not result in detectable aerosolization of SARS-CoV-2.
Conclusions:
- The reprocessing of medical devices, including respiratory equipment, does not appear to significantly aerosolize SARS-CoV-2.
- Medical masks are likely sufficient personal protective equipment for staff in sterile processing departments during the COVID-19 pandemic.
- Further research could explore long-term implications and other reprocessing methods.
More Related Videos
Related Concept Videos
Transmission-based Precautions II: Airborne and Protective Environment
Airborne precautions:
Use airborne precautions when treating patients known or suspected to have diseases that spread through the air—for example, tuberculosis or measles. These organisms are present in smaller droplets expelled by an infected person and...
Suctioning the Nasopharyngeal Airway
Equipment Required
Tracheostomy Suctioning II: Procedure
Suctioning the Oropharyngeal Airway
After assembling the equipment, the nurse should practice hand hygiene and don appropriate PPE according to infection control guidelines to avoid the...
Standard Precaution
Hand hygiene is the most crucial means to prevent the transmission of disease. Employers are legally required to provide their workers with personal protective equipment (PPE) to minimize exposure or contact with...

