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Expression of the miR-302/367 microRNA cluster is regulated by a conserved long non-coding host-gene

Karim Rahimi1, Annette Christine Füchtbauer2, Fardin Fathi3

  • 1Department of Molecular Biology and Genetics, Aarhus University, C.F. Møllers Alle 3, 8000, Aarhus C, Denmark. karim@mbg.au.dk.

Scientific Reports
|May 28, 2021
PubMed

Insights

This study reveals how the mouse miR-302 host gene is regulated and processed. Its regulatory elements are conserved in humans, suggesting a broader role in microRNA gene regulation.

Area of Science:

  • Molecular Biology
  • Genetics
  • Developmental Biology

Background:

  • MicroRNAs (miRNAs) are key regulators of cellular functions.
  • The miR-302/367 cluster is crucial for inducing and maintaining pluripotency.
  • The transcriptional control and processing of the miR-302 host gene are not fully understood.

Purpose of the Study:

  • To investigate the transcriptional control of the mouse miR-302 host gene.
  • To analyze the processing mechanisms of the mmu-miR-302 host gene.
  • To explore the evolutionary conservation of the miR-302 gene structure and regulatory elements.

Main Methods:

  • Analysis of gene structure and alternative splicing.
  • Identification of regulatory sequences and transcription factor binding sites.
  • Comparative genomics between mouse and human sequences.

Main Results:

  • The mmu-miR-302 host gene undergoes alternative splicing, polyadenylation, and nuclear export.
  • Regulatory sequences extend over 2 kb upstream, containing conserved binding sites for transcription factors.
  • The gene structure and regulatory elements show high conservation between mouse and human.

Conclusions:

  • The miR-302 host gene's regulation involves complex transcriptional control and processing.
  • Conserved regulatory elements suggest a significant role in miR-302 expression.
  • Long non-coding RNA genes may have an under-recognized function in regulating microRNA transcription.

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