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Isolation of Mouse Interstitial Valve Cells to Study the Calcification of the Aortic Valve In Vitro
Published on: May 10, 2021
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Predicting the Key Genes Involved in Aortic Valve Calcification Through Integrated Bioinformatics Analysis
Dinghui Wang1, Tianhua Xiong1, Wenlong Yu1
1Department of Cardiology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China.
Frontiers in Genetics
|May 28, 2021
Summary
Calcific aortic valve disease (CAVD) lacks effective treatments. This study identifies key chemokines and immune cells, like macrophages and B cells, involved in CAVD progression, offering potential therapeutic targets.
Area of Science:
- Cardiovascular Research
- Immunology
- Bioinformatics
Background:
- Calcific aortic valve disease (CAVD) is the most prevalent valvular heart disease globally.
- Current treatments for CAVD are limited in delaying disease progression.
- The precise molecular mechanisms underlying aortic valve calcification remain largely unknown.
Purpose of the Study:
- To identify differentially expressed genes and key molecular players in CAVD.
- To investigate the role of immune cell infiltration in the pathogenesis of CAVD.
- To uncover potential biomarkers and therapeutic targets for CAVD.
Main Methods:
- Utilized gene expression datasets (GSE12644, GSE51472) from the GEO database.
- Applied bioinformatics analyses including Gene Ontology (GO), KEGG pathway analysis, and protein-protein interaction (PPI) network construction.
- Performed immune infiltration analysis using CIBERSORT to assess immune cell proportions in CAVD.
Main Results:
- Identified 144 differentially expressed genes (DEGs) in CAVD, with 49 upregulated and 95 downregulated.
- GO analysis revealed enrichment in immune response, inflammation, and apoptosis pathways.
- KEGG analysis highlighted the chemokine signaling pathway, cytokine-cytokine receptor interaction, and PI3K-Akt signaling pathway.
- Key genes identified include chemokines CXCL13, CCL19, CCL8, CXCL8, CXCL16, MMP9, CCL18, CXCL5, VCAM1, and PPBP.
- Macrophages constituted the largest immune cell proportion, with increased M0, memory B cells, and plasma cells, and decreased naive B cells, activated NK cells, and M2 macrophages in CAVD valves.
Conclusions:
- Chemokines CXCL13, CXCL8, CXCL16, CXCL5, CCL19, CCL8, and CCL18 are significant markers for aortic valve disease.
- Macrophages M0, plasma cells, memory B cells, naive B cells, activated NK cells, and macrophages M2 are implicated in the development and progression of aortic valve stenosis.
- These identified chemokines and immune cells likely interact to regulate calcification in CAVD.
