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Updated: Nov 4, 2025
A Murine Model of Fetal Exposure to Maternal Inflammation to Study the Effects of Acute Chorioamnionitis on Newborn Intestinal Development
Published on: June 24, 2020
MicroRNAs miR-4535 and miR-1915-5p in amniotic fluid as predictive biomarkers for chorioamnionitis
Chihiro Kiyoshima1, Naoto Shirasu2,3, Daichi Urushiyama1
1Department of Obstetrics & Gynecology, Fukuoka University, Fukuoka, Japan.
Background:
This study was performed to investigate the clinical significance of miR-4535 and miR-1915-5p in severe chorioamnionitis.
Materials & Methods:
Amniotic fluid samples from 37 patients with severe chorioamnionitis were subjected to miRNA array analysis and ddPCR™. Diagnostic values were assessed using the receiver operating characteristic curve. The patients were separated into three groups according to Blanc's criteria.
Results:
The expression of miR-4535 and miR-1915-5p was significantly correlated with the copy number of 16S rDNA, had extremely high diagnostic accuracy for severe chorioamnionitis, and was linked to maternal and fetal inflammation.
Conclusion:
miR-4535 and miR-1915-5p serve as promising biomarkers for the diagnosis of severe chorioamnionitis.
Insights
Two microRNAs, miR-4535 and miR-1915-5p, show high diagnostic accuracy for severe chorioamnionitis. These biomarkers are linked to maternal and fetal inflammation, aiding in diagnosis.
Area of Science:
- Reproductive Medicine
- Molecular Diagnostics
- MicroRNA Research
Background:
- Severe chorioamnionitis poses significant risks to maternal and fetal well-being.
- Accurate and early diagnosis is crucial for effective management.
Purpose of the Study:
- To investigate the clinical significance of specific microRNAs (miRNAs) in severe chorioamnionitis.
- To evaluate miR-4535 and miR-1915-5p as potential diagnostic biomarkers.
Main Methods:
- Amniotic fluid samples were collected from 37 patients diagnosed with severe chorioamnionitis.
- MicroRNA expression was analyzed using miRNA array and digital droplet PCR (ddPCR).
- Diagnostic accuracy was assessed via receiver operating characteristic (ROC) curve analysis.
Main Results:
- Expression levels of miR-4535 and miR-1915-5p were significantly correlated with 16S rDNA copy numbers.
- These miRNAs demonstrated exceptionally high diagnostic accuracy for severe chorioamnionitis.
- Elevated miRNA expression was associated with maternal and fetal inflammatory markers.
Conclusions:
- miR-4535 and miR-1915-5p are identified as promising biomarkers for diagnosing severe chorioamnionitis.
- These miRNAs offer a potential tool for improved clinical management of this condition.
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