[Identification of active components of Dendrobium inhibit growth of gastric cancer cells based on component-activity
Jia-Ni Liu1, Feng-Zhong Wang1, Jia-Meng Liu1
1Key Laboratory of Agro-Products Quality and Safety in Harvest, Storage, Transportation, Management and Control, Ministry of Agriculture and Rual Affairs, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences Beijing 100193, China.
Abstract:
Three cancer cell lines including gastric cancer SGC-7901, HGC-27, and MGC-803 cells were employed to evaluate the bioactivity of seven Dendrobium species. Simultaneously, these Dendrobium species were assessed with UPLC-Q-TOF-MS, and 504 common peaks were found. Based on the hypothesis that biological effects varied with differences in components, multivariate relevance analysis for chemical component-activity relationship of Dendrobium, including grey relation(GRA) and partial least squares(PLS) analysis were performed to evaluate the contribution of each identified component. The target peaks were identified by standards toge-ther with databases of Dendrobium, Nature Chemistry, MassBank, etc. Finally, four active components, including 3,5,9-trihydroxy-23-methylergosta-7,22-dien-6-one, diacylglycerol(14∶1/22∶6/0∶0), pipercitine, and 22-tricosenoic acid, might have negative effect on the growth of gastric cancer cells.
Insights
This study investigated the anti-gastric cancer potential of seven Dendrobium species. Four compounds were identified as potentially inhibiting gastric cancer cell growth, offering new therapeutic leads.
Area of Science:
- Phytochemistry
- Pharmacology
- Cancer Research
Background:
- Dendrobium species are traditionally used in medicine.
- Gastric cancer remains a significant global health challenge.
- Understanding the chemical constituents and bioactivity of Dendrobium is crucial for therapeutic development.
Purpose of the Study:
- To evaluate the bioactivity of seven Dendrobium species against gastric cancer cell lines.
- To identify active chemical components responsible for the observed bioactivity.
- To establish a chemical component-activity relationship using multivariate analysis.
Main Methods:
- Utilized three human gastric cancer cell lines (SGC-7901, HGC-27, MGC-803).
- Employed Ultra-Performance Liquid Chromatography-Quadrupole Time-of-Flight Mass Spectrometry (UPLC-Q-TOF-MS) for chemical profiling.
- Performed multivariate relevance analyses, including Grey Relation Analysis (GRA) and Partial Least Squares (PLS), to link chemical components to bioactivity.
Main Results:
- Identified 504 common peaks across the seven Dendrobium species.
- Four compounds, 3,5,9-trihydroxy-23-methylergosta-7,22-dien-6-one, diacylglycerol(14∶1/22∶6/0∶0), pipercitine, and 22-tricosenoic acid, were identified as potentially active.
- These compounds demonstrated a negative effect on the proliferation of gastric cancer cells.
Conclusions:
- The study successfully linked specific chemical components of Dendrobium to anti-gastric cancer activity.
- Identified compounds represent potential novel therapeutic agents for gastric cancer treatment.
- Further research is warranted to elucidate the precise mechanisms of action and optimize these compounds for clinical application.
