Related Experiment Video
Updated: Nov 4, 2025
11:53
Cecal Ligation Puncture Procedure
Published on: May 7, 2011
55.5K
Cecal Ligation and Puncture.
Susanne Drechsler1, Marcin Osuchowski2
1Ludwig Boltzmann Institute for Experimental and Clinical Traumatology in the AUVA Research Center, Vienna, Austria.
Methods in Molecular Biology (Clifton, N.J.)
|May 28, 2021
Summary
The cecal ligation and puncture (CLP) model simulates abdominal sepsis in rodents by creating a polymicrobial infection. This "gold standard" model requires careful management of analgesia, antibiotics, and fluids for clinical relevance.
Area of Science:
- Surgical models
- Infectious disease research
- Rodent models
Background:
- Cecal ligation and puncture (CLP) is the established standard rodent model for inducing abdominal sepsis.
- This model replicates a polymicrobial infectious focus with continuous leakage within the abdominal cavity.
- It is crucial for studying the pathophysiology and potential treatments of sepsis.
Purpose of the Study:
- To describe the standard procedure for the cecal ligation and puncture (CLP) rodent model.
- To highlight factors influencing CLP severity and clinical relevance.
- To emphasize critical care interventions for enhancing the model's applicability.
Main Methods:
- Surgical induction of sepsis via cecal ligation and puncture under anesthesia and analgesia.
- Manipulation of ligation length and needle size to modulate infection severity.
- Administration of supportive care including analgesia, antibiotics, and fluid resuscitation.
Main Results:
- The CLP procedure reliably establishes a polymicrobial infectious focus.
- Clinical signs of systemic bacterial infection develop within 24 hours post-procedure.
- Adjustable parameters allow for controlled induction of sepsis of varying severity.
Conclusions:
- The CLP model is a valuable tool for abdominal sepsis research.
- Optimizing surgical technique and supportive care enhances the model's clinical relevance.
- This model facilitates the investigation of sepsis pathogenesis and therapeutic strategies.
