Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Developing a prioritized research agenda for necrotizing enterocolitis.

Journal of perinatology : official journal of the California Perinatal Association·2026

Same author

Urolithin A activates aryl hydrocarbon receptor-NLRP6-mediated pathways in intestinal epithelial cells to modulate mucosal immunity and strengthen gut barrier integrity.

Nature communications·2026

Same author

Compressing neonatal-perinatal medicine fellowship training: a critical appraisal of the American Board of Pediatrics proposed 2-year pathway.

Journal of perinatology : official journal of the California Perinatal Association·2026

Same author

Urolithin A attenuates inflammation and enhances barrier integrity in an experimental NEC-in-a-Dish model.

Pediatric research·2026

Same author

Non-Mendelian inheritance of DNA methylation patterns in mice.

Nature genetics·2026

Same author

The complex interaction of enteral feeding in the pathogenesis of necrotizing enterocolitis.

Seminars in perinatology·2026

Search research articles

Related Experiment Video

Updated: Nov 4, 2025

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis

Published on: November 30, 2021

A Mouse Model of Necrotizing Enterocolitis.

Belgacem Mihi¹, Wyatt E Lanik¹, Qingqing Gong¹

¹Division of Newborn Medicine, Department of Pediatrics, Washington University School of Medicine, St. Louis Children's Hospital, St. Louis, MO, USA.

Methods in Molecular Biology (Clifton, N.J.)

|May 28, 2021

Summary

This summary is machine-generated.

Necrotizing enterocolitis (NEC), a severe intestinal disease in premature infants, can now be studied using a new mouse model. This model mimics key features of human NEC, aiding research into treatments.

Keywords:

Enteric bacteria Intestinal inflammation Mucosal barrier Necrotizing enterocolitis Neonatal mice

More Related Videos

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Published on: July 28, 2023

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

Published on: April 10, 2019

Related Experiment Videos

Last Updated: Nov 4, 2025

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis

A Neonatal BALB/c Mouse Model of Necrotizing Enterocolitis

Published on: November 30, 2021

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Author Spotlight: Enhancing Understanding and Treatment Strategies with the NEC-on-a-Chip Model

Published on: July 28, 2023

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis

Published on: April 10, 2019

Area of Science:

Neonatal Medicine
Gastroenterology
Pathology

Background:

Necrotizing enterocolitis (NEC) is a critical gastrointestinal emergency primarily affecting very low birth weight premature infants.
The disease involves intestinal barrier dysfunction, leading to necrosis and potentially fatal multisystem organ failure.
Understanding NEC's molecular basis is crucial for developing effective therapeutic strategies.

Purpose of the Study:

To present a detailed protocol for a novel mouse model of NEC.
To ensure the model recapitulates key histological and transcriptional characteristics of human NEC.
To facilitate further research into NEC pathogenesis and treatment.

Main Methods:

Development of a specific protocol to induce NEC in newborn mice.
Histological analysis to evaluate intestinal tissue damage.
Transcriptional profiling to compare gene expression patterns with human NEC cases.

Main Results:

The established mouse model successfully replicated major histological features observed in human NEC.
Transcriptional analysis revealed similarities between the mouse model and human NEC, indicating shared molecular pathways.
The protocol provides a reproducible method for generating a relevant NEC animal model.

Conclusions:

The developed mouse model serves as a valuable tool for investigating NEC.
This model enables deeper exploration of NEC's molecular mechanisms and the testing of new therapies.
Further research using this model can advance the clinical management of NEC in premature infants.