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Updated: Nov 4, 2025
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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
Published on: November 16, 2021
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Identification of ILC2 in the Lung Using Flow Cytometry
1State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral Diseases, Department of Orthodontics, West China Hospital of Stomatology, Sichuan University, Chengdu, P. R. China.
Methods in Molecular Biology (Clifton, N.J.)
|May 28, 2021
Summary
Group 2 innate lymphoid cells (ILC2) are crucial in lung immunity and sepsis-induced injury. This study presents a new flow cytometry method for easier ILC2 detection in mouse lungs, aiding sepsis research.
Area of Science:
- Immunology
- Cell Biology
Background:
- Group 2 innate lymphoid cells (ILC2) are key immune cells in the lung, influencing both innate and adaptive immunity.
- ILC2 play significant roles in lung injury associated with sepsis.
- Understanding ILC2 pathobiology may lead to novel therapeutic strategies for sepsis.
Purpose of the Study:
- To develop and describe a method for detecting ILC2 in mouse lungs.
- To overcome the challenges associated with identifying ILC2 due to multiple marker requirements.
Main Methods:
- Utilized flow cytometry for ILC2 detection.
- Focused on ILC2 identification within the mouse lung environment.
Main Results:
- Successfully described a method for detecting ILC2 in mouse lungs using flow cytometry.
- The method aims to simplify the challenging identification process of ILC2.
Conclusions:
- The developed method facilitates ILC2 detection in mouse lungs.
- This advancement can support further research into ILC2's role in lung injury and sepsis.
