PAN2HGENE-tool for comparative analysis and identifying new gene products

Area of Science:

• Genomics and Bioinformatics
• Computational Biology

Background:

• Next-generation sequencing (NGS) has advanced omics research, providing gene content insights.
• Comparative analysis is crucial for gene function inference, ortholog/paralog identification, and evolutionary studies.
• Existing comparative analysis tools are often limited to complete genomes.

Purpose of the Study:

• To introduce PAN2HGENE, a computational tool for automated comparative analysis of gene products.
• To address the limitation of existing tools by enabling analysis of both complete and draft genomes.
• To identify novel gene products and assess their impact on pangenome alpha value behavior.

Main Methods:

• Utilized PAN2HGENE for automated comparative analysis on genomic data.
• Applied the tool to identify gene products potentially missing from original genome sequences.
• Evaluated the impact of identified gene products on pangenome alpha value calculations.

Main Results:

• PAN2HGENE successfully identified new gene products.
• The identification of these products altered the pangenome's alpha value behavior.
• The original genomic sequences remained unaltered during the analysis.
• PAN2HGENE demonstrated efficiency and ease of use via its graphical interface.

Conclusions:

• PAN2HGENE is an efficient computational tool for comparative genomic analysis.
• It effectively identifies missing gene products in draft and complete genomes.
• The tool offers a user-friendly alternative for enhancing pangenome studies.