Assaying of Cu²⁺ with near-infrared l-cysteine-capped CdSeTe/CdS quantum dots and its effect on cell imaging

Near-infrared (NIR) core-shell CdSeTe/CdS quantum dots (QDs) modified with l-cysteine were synthesized in aqueous solution. The QDs had a special NIR-emitting spectrum, high fluorescence stability and low cytotoxicity. In addition, they exhibited an obvious fluorescence quenching when Cu²⁺ was present. An NIR nanosensor was prepared for rapidly, sensitively, and selectively determining Cu²⁺ in solution quantitatively and monitoring the changes in Cu²⁺ in cells with fluorescence imaging in a semiquantitative way. The linear relationship between the relative fluorescence intensity (F₀ /F) and the concentration of Cu²⁺ from 5.12 × 10^-8  M to 2.56 × 10^-5  M in solution was observed using an NIR fluorescence spectrophotometer with R² equal to 0.9958. Moreover, in the experiment with the fluorescence microscope, F₀ /F versus the concentration of Cu²⁺ from 5.00 × 10^-8  M to 7.68 × 10^-6  M also showed a good linear relationship with R² equal to 0.9817. Practical water sample ion detecting experiments had good accuracy and recovery rates. Cell experiments showed that the NIR imaging intensity of cells was inversely proportional to the concentration of copper ions, therefore NIR QDs have great potential for detection of metal ions in solution and in cells.