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Related Experiment Video

Updated: Nov 4, 2025

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Cas14a1-mediated nucleic acid detectifon platform for pathogens.

Xiaolin Ge1, Tian Meng1, Xiao Tan2

  • 1State Key Laboratory of Marine Resource Utilization in South China Sea, Marine College, Key Laboratory of Tropical Biological Resources of Ministry of Education, School of Life and Pharmaceutical Sciences, Hainan University, 56 Renmin Road, Haikou, 570228, China.

Biosensors & Bioelectronics
|May 28, 2021
PubMed
Summary

A novel CRISPR-Cas14a1 biosensing platform offers sensitive and specific bacterial detection. This fluorescence tool accurately identifies pathogens in milk, advancing genomic diagnostics for food safety.

Keywords:
CRISPR-CasCas14a1Nucleic acid detection platformPathogenic detection

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Genomic Diagnostics

Background:

  • CRISPR-Cas systems are emerging genomic diagnostic tools for pathogen detection.
  • Existing Cas effectors often exhibit limited single-strand DNA recognition and protospacer adjacent motif constraints, hindering universal application.
  • There is a need for improved CRISPR-based biosensors with enhanced sensitivity and specificity for diverse pathogen identification.

Purpose of the Study:

  • To develop a highly sensitive and specific fluorescence-based biosensing tool for bacterial detection.
  • To create a CRISPR-Cas14a1-mediated nucleic acid detection platform (CMP) overcoming limitations of current systems.
  • To demonstrate the utility of the CMP technique for identifying various pathogens in complex matrices like milk samples.

Main Methods:

  • Utilized the unique collateral cleavage activity of the Cas14a1 effector.
  • Integrated the Cas14a1-mediated nucleic acid detection platform (CMP) with molecular amplification techniques.
  • Developed a fluorescence-based assay for rapid nucleic acid detection.
  • Applied the developed technique to detect different bacterial species in milk samples.

Main Results:

  • The developed CRISPR-Cas based bioanalysis technique demonstrated high sensitivity and specificity.
  • The Cas14a1-mediated nucleic acid detection platform (CMP) enabled fast and accurate detection of nucleic acids.
  • The technique successfully identified different species of pathogens in milk samples with excellent accuracy.
  • The system overcomes limitations in single-strand DNA recognition and protospacer adjacent motif constraints.

Conclusions:

  • The Cas14a1-mediated nucleic acid detection platform (CMP) offers a sensitive and specific fluorescence tool for bacterial detection.
  • This CRISPR-Cas based bioanalysis technique presents a promising platform for genomic diagnostics in biomedicine and food safety.
  • The developed method provides an accurate and efficient approach for pathogen identification in real-world samples.