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Measurement of human corticosteroid binding globulin by enzyme-linked immunoassay
V E Fantl1, D Y Wang, C I Mockridge
1Imperial Cancer Research Fund, Lincoln's Inn Fields, London, England.
Journal of Steroid Biochemistry
|August 1, 1988
Abstract:
Thirteen monoclonal antibodies have been raised against corticosteroid binding globulin (CBG). From four of those with highest affinity for the antigen, two were selected for development of a sandwich enzyme-linked immunoassay (ELISA). The sensitivity of the assay was such that 0.7 fmol CBG could be detected. Levels of the binding protein in men (740 +/- 67 nmol/l) and women (690 +/- 103 nmol/l) were not significantly different, while those found during the third trimester of pregnancy (1500 +/- 423 nmol/l) were approximately twice these levels. CBG denatured by heating to 60 degrees C could not be detected by the ELISA.