Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

788
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
788
Homologous Recombination02:31

Homologous Recombination

57.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
57.9K
CRISPR01:59

CRISPR

54.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
54.0K
CRISPR and crRNAs02:53

CRISPR and crRNAs

18.0K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
18.0K
Improving Translational Accuracy02:07

Improving Translational Accuracy

12.1K
Base complementarity between the three base pairs of mRNA codon and the tRNA anticodon is not a failsafe mechanism. Inaccuracies can range from a single mismatch to no correct base pairing at all. The free energy difference between the correct and nearly correct base pairs can be as small as 3 kcal/ mol. With complementarity being the only proofreading step, the estimated error frequency would be one wrong amino acid in every 100 amino acids incorporated. However, error frequencies observed in...
12.1K
Improving Translational Accuracy02:07

Improving Translational Accuracy

3.2K
3.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NanoporeDB: A Structural Resource Of Multimeric Protein Nanopores For Single-Molecule Sensing.

GigaScience·2026
Same author

Lamprey 3D single-cell transcriptomics reveals ancestral and specialized features of the vertebrate brain.

Science (New York, N.Y.)·2026
Same author

Single nucleus RNA sequencing reveals testosterone replacement therapy-associated multicellular remodelling of skeletal muscle in hypogonadal men with Klinefelter Syndrome.

The Journal of clinical endocrinology and metabolism·2026
Same author

Genomic landscape of the human vaginal microbiome is linked to host genetics and population of origin.

Nature genetics·2026
Same author

Spatiotemporal transcriptome atlas of human embryos after gastrulation.

Nature·2026
Same author

4D single-cell spatial transcriptomics reveals dynamic morphogenetic gradients and regenerative domains in planarians.

GigaScience·2026

Related Experiment Video

Updated: Nov 4, 2025

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

10.0K

Enhancing CRISPR-Cas9 gRNA efficiency prediction by data integration and deep learning.

Xi Xiang1,2,3,4, Giulia I Corsi5, Christian Anthon5

  • 1Lars Bolund Institute of Regenerative Medicine, Qingdao-Europe Advanced Institute for Life Sciences, BGI-Qingdao, Qingdao, China.

Nature Communications
|May 29, 2021
PubMed
Summary
This summary is machine-generated.

This study introduces CRISPRon, a deep learning model for predicting CRISPR guide RNA (gRNA) efficiency. CRISPRon offers improved accuracy for SpCas9 gRNA design, advancing CRISPR technology applications.

More Related Videos

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

15.2K
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
11:31

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Published on: April 2, 2016

14.4K

Related Experiment Videos

Last Updated: Nov 4, 2025

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution
11:37

Using Sniper-Cas9 to Minimize Off-target Effects of CRISPR-Cas9 Without the Loss of On-target Activity Via Directed Evolution

Published on: February 26, 2019

10.0K
CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy
08:22

CRISPR/Cas9-mediated Targeted Integration In Vivo Using a Homology-mediated End Joining-based Strategy

Published on: March 12, 2018

15.2K
Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells
11:31

Generating CRISPR/Cas9 Mediated Monoallelic Deletions to Study Enhancer Function in Mouse Embryonic Stem Cells

Published on: April 2, 2016

14.4K

Area of Science:

  • Molecular Biology
  • Bioinformatics
  • Genetics Engineering

Background:

  • Accurate prediction of CRISPR guide RNA (gRNA) on-target efficiency is crucial for effective genome editing.
  • Existing gRNA activity data and modeling approaches have limitations in prediction accuracy.

Purpose of the Study:

  • To generate a large dataset of on-target SpCas9 gRNA activity.
  • To develop and validate a deep learning model (CRISPRon) for enhanced gRNA efficiency prediction.
  • To provide a user-friendly webserver for gRNA design.

Main Methods:

  • Generated 10,592 new on-target SpCas9 gRNA activity data points.
  • Integrated new data with published datasets, totaling 23,902 gRNAs.
  • Trained a deep learning model, CRISPRon, utilizing the comprehensive gRNA dataset.

Main Results:

  • CRISPRon demonstrated significantly higher prediction performance compared to existing tools on four independent test datasets.
  • The model's accuracy was validated on data not used during its training.
  • An interactive webserver and standalone software were developed for CRISPRon.

Conclusions:

  • CRISPRon provides more accurate gRNA efficiency predictions than current tools.
  • The developed tool and webserver facilitate improved CRISPR-based applications.
  • This work advances the field of CRISPR genome editing through enhanced predictive modeling.