Related Experiment Video

Updated: Nov 4, 2025

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells
10:33

Assessing Cell Viability and Death in 3D Spheroid Cultures of Cancer Cells

Published on: June 16, 2019

28.6K

Effect of M2 Macrophage-Derived Soluble Factors on Proliferation and Apoptosis of SH-SY5Y Cells

I M Rashchupkin1, A A Maksimova2, L V Sakhno2

  • 1Research Institute of Fundamental and Clinical Immunology, Novosibirsk, Russia. iwwwanbets@mail.ru.

Bulletin of Experimental Biology and Medicine
|May 29, 2021
PubMed

Insights

M2 macrophages, crucial for neuroregeneration, promote neural cell proliferation and reduce apoptosis. These findings highlight the significant neuroregenerative potential of M2 macrophages via soluble factors.

Area of Science:

  • Neuroscience
  • Immunology
  • Cell Biology

Background:

  • Macrophages are critical regulators of neuroregeneration.
  • M2 macrophages are known for their role in tissue repair and immune modulation.
  • Understanding their specific neuroregenerative capabilities is essential for therapeutic development.

Purpose of the Study:

  • To evaluate the neuroregenerative potential of M2 macrophages.
  • To assess the impact of M2 macrophages polarized via IL-4 (M2a) and efferocytosis under low serum conditions (M2(LS)) on neuronal cell behavior.
  • To investigate the effects on SH-SY5Y cell proliferation and apoptosis under growth factor-deficient conditions.

Main Methods:

  • Culturing of SH-SY5Y neuroblastoma cells.
  • Polarization of M2 macrophages using IL-4 (M2a) and efferocytosis in low serum (M2(LS)).
  • Treatment of SH-SY5Y cells with conditioned media from polarized M2 macrophages.
  • Assessment of cell proliferation and apoptosis rates.

Main Results:

  • Conditioned media from both M2(LS) and M2a(IL-4) macrophages significantly stimulated SH-SY5Y cell proliferation.
  • Soluble factors from M2(LS) and M2a(IL-4) macrophages reduced early apoptosis in SH-SY5Y cells.
  • The protective effect against apoptosis by M2(LS) macrophages was observed at earlier time points.

Conclusions:

  • M2 macrophages possess substantial neuroregenerative potential.
  • This potential is mediated through soluble factors secreted by the macrophages.
  • M2 macrophages promote neuronal survival and proliferation, suggesting therapeutic applications in neurodegenerative conditions.
Keywords:
apoptosishuman macrophagesneural stem cellsproliferation

More Related Videos

Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity09:31

Drug-Induced Senescence in Liver Cells Promotes M2 Macrophage Polarization: Implications for Tyrosine Kinase Inhibitor-Associated Hepatotoxicity

Published on: October 17, 2025

90
Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports07:44

Studying the Effects of Tumor-Secreted Paracrine Ligands on Macrophage Activation using Co-Culture with Permeable Membrane Supports

Published on: November 28, 2019

7.7K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K