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Enzyme-Linked Immunosorbent Assay

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Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Development of a Lateral Flow Immunochromatographic Strip for Rapid and Quantitative Detection of Small Molecule Compounds

Published on: November 13, 2021

SERS-based test strips: Principles, designs and applications.

Luyang Wang¹, Xiaokun Wang², Lu Cheng¹

¹College of Food Science and Engineering, Ocean University of China, 5 Yushan Road, Qingdao, 266003, China.

Biosensors & Bioelectronics

|May 29, 2021

Summary

This summary is machine-generated.

Surface-enhanced Raman scattering (SERS) test strips offer ultrasensitive detection for disease biomarkers, nucleic acids, and toxins. This review explores their design, working principles, and point-of-care testing applications, enhancing diagnostic capabilities.

Keywords:

Lateral flow assay Medical diagnosis Nanoparticles Point-of-care testing Toxin detection Vertical flow assay

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Area of Science:

Analytical Chemistry
Biomedical Engineering
Nanotechnology

Background:

Conventional test strips provide simple, rapid diagnostics but lack sensitivity and quantitative analysis.
Surface-enhanced Raman scattering (SERS) offers a pathway to ultrasensitive and multiplexed detection.
Combining SERS with test strips creates a powerful platform for advanced point-of-care testing (POCT).

Purpose of the Study:

To review the working principles, design strategies, and POCT applications of SERS-based test strips.
To highlight advancements in SERS test strip technology for enhanced detection performance.
To discuss the application of SERS test strips in diagnosing disease biomarkers, nucleic acids, and toxins.

Main Methods:

Introduction to lateral and vertical flow test strip principles.
Discussion of various strategies for optimizing SERS-based test strip design.
Review of SERS tag design, sensitivity, and analytical applicability in diagnostics.

Main Results:

SERS-based test strips enable ultrasensitive and multiplexed detection of various analytes.
Optimized designs improve the sensitivity and analytical performance of SERS test strips.
Successful applications demonstrated in the detection of disease biomarkers, nucleic acids, and toxins.

Conclusions:

SERS-based test strips represent a significant advancement over conventional methods for POCT.
The technology offers a promising future for sensitive and quantitative disease diagnosis.
Further research directions include refining SERS tag design and expanding analytical applications.