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A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Optimization of aerobic dynamic discharge process for very rapid enrichment of polyhydroxyalkanoates-accumulating
Daisuke Inoue1, Atsushi Fukuyama1, Yu Ren1
1Division of Sustainable Energy and Environmental Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
Abstract:
The aerobic dynamic discharge (ADD) process has the potential to reduce the enrichment period of polyhydroxyalkanoates (PHA)-accumulating bacteria in PHA production using mixed microbial cultures (MMCs). This study aimed to efficiently enrich PHA-accumulating bacteria from activated sludge within a fixed period of 2 d by optimizing operating conditions of the ADD process. Based on the results, enrichment with separate feeding of carbon and nutrients in the feast and famine phases, respectively, and a settling duration of 10 min after the feast phase in the sequencing batch cycle for 12 h was found to be optimal. The MMC enriched at optimum conditions could store as much as 68.4 wt% of PHA. Dechloromonas and Zoogloea were identified as potential PHA-accumulating bacteria responsible for enhancing PHA accumulation ability in the enriched MMC. The optimized ADD process will facilitate the consecutive use of daily generated waste activated sludge for PHA production.
