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Cloning and expression of two human genes encoding calcium-binding proteins that are regulated during myeloid

E Lagasse1, R G Clerc

  • 1Department of Biotechnology, CIBA-GEIGY, Basel, Switzerland.

Molecular and Cellular Biology
|June 1, 1988
PubMed

Insights

Myeloid-related proteins (MRP8 and MRP14) are key in inflammation. Their genes

Area of Science:

  • Cellular and Molecular Biology
  • Immunology
  • Biochemistry

Background:

  • Chronic inflammatory processes and the role of macrophages remain poorly understood.
  • Myeloid-related proteins (MRP8 and MRP14) are expressed in inflammatory macrophages but not in normal tissue macrophages.

Purpose of the Study:

  • To characterize the MRP8 and MRP14 genes and their expression patterns.
  • To investigate the cis-acting elements regulating tissue-specific MRP gene expression.

Main Methods:

  • Cloning of human MRP8 and MRP14 genes.
  • Analysis of gene organization and homology.
  • Transient expression assays in HL60 leukemia cells.

Main Results:

  • MRP8 and MRP14 mRNAs are specifically expressed in human myeloid cells and regulated during differentiation.
  • MRP8 and MRP14 genes share similar organization and belong to a novel calcium-binding protein subfamily.
  • Tissue-specific regulatory elements for MRP gene expression are located within the cloned gene loci.

Conclusions:

  • MRP8 and MRP14 are specifically regulated in myeloid cells during differentiation.
  • The cloned MRP gene loci contain the necessary cis-acting elements for tissue-specific expression.

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