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Activated AMPK promoted the decrease of lactate production in rat Sertoli cells exposed to Zearalenone.

Peirong Cai1, Zhiheng Feng2, Nannan Feng2

  • 1College of Veterinary Medicine, Yangzhou University, 12 Wenhui East Road, Yangzhou 225009, Jiangsu, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, Jiangsu, China; Joint International Research Laboratory of Agriculture and Agri-Product Safety of the Ministry of Education of China, Yangzhou University, Yangzhou 225009, Jiangsu, China.

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Zearalenone mycotoxin disrupts Sertoli cell energy by affecting AMPK, leading to reproductive damage. Modulating AMPK impacts lactate and pyruvate production, crucial for germ cell energy supply.

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Area of Science:

  • Reproductive Toxicology
  • Cellular Metabolism
  • Endocrinology

Background:

  • Zearalenone (ZEA) is a ubiquitous mycotoxin causing reproductive toxicity.
  • Sertoli cells (SCs) are vital for germ cell development and energy supply.
  • AMP-activated protein kinase (AMPK) regulates cellular energy metabolism.

Purpose of the Study:

  • To investigate the regulatory role of AMPK in ZEA-induced alterations of lactate production in SCs.
  • To elucidate the impact of AMPK activation and inhibition on SCs exposed to ZEA.

Main Methods:

  • ZEA-treated SCs were exposed to AICAR (AMPK activator) or Compound C (AMPK inhibitor).
  • Intracellular lactate and pyruvate production were measured.
  • Expression of glycolysis-related genes and lactate production proteins was analyzed.

Main Results:

  • ZEA disrupted SC energy balance and activated P-AMPK, inhibiting lactate and pyruvate production.
  • AMPK inhibition increased lactate and pyruvate via enhanced glycolysis gene/protein expression.
  • AMPK activation suppressed lactate and pyruvate by downregulating glycolysis gene/protein expression.

Conclusions:

  • ZEA-induced P-AMPK activation impairs SC energy metabolism, reducing lactate and pyruvate.
  • This energy deficit compromises SC support for spermatogenesis, contributing to reproductive system damage.
  • Targeting AMPK may offer a strategy to mitigate ZEA's reproductive toxicity.