Nicotinamide Riboside Enhances Endothelial Precursor Cell Function to Promote Refractory Wound Healing Through...

Area of Science:

• Biomedical Science
• Regenerative Medicine
• Metabolic Disease Research

Background:

• Diabetic wound healing is impaired by poor vascularization.
• Enhancing endothelial precursor cell (EPC) function is a therapeutic goal for diabetic wounds.
• Nicotinamide riboside (NR) is investigated for its potential effects on EPCs.

Purpose of the Study:

• To investigate the effect of nicotinamide riboside (NR) on endothelial precursor cell (EPC) function.
• To determine if NR supplementation improves wound healing in diabetes mellitus (DM).
• To elucidate the molecular mechanisms underlying NR's action on EPCs.

Main Methods:

• Treatment of db/db mice with NR-supplemented food.
• Assessment of wound healing rates and angiogenesis.
• Analysis of EPC numbers, function (tube formation, adhesion), and molecular markers (Sirt1, p-AMPK, PGC-1α) in vitro and in vivo.
• Gene knockdown and pathway inhibition studies (Sirt1, AMPK).

Main Results:

• NR supplementation significantly increased wound healing rates and angiogenesis in diabetic mice.
• NR increased the number and functional capacity (tube formation, adhesion) of EPCs.
• NR enhanced Sirt1 and p-AMPK expression while reducing acetylated PGC-1α in EPCs.
• The Sirt1-AMPK pathway was identified as crucial for NR-mediated enhancement of EPC function.

Conclusions:

• Oral nicotinamide riboside (NR) enhances endothelial precursor cell (EPC) function.
• NR promotes diabetic wound healing through the Sirt1-AMPK signaling pathway.
• NR supplementation represents a promising therapeutic strategy for diabetic wound complications.