Related Experiment Video
Updated: Nov 4, 2025
08:01
Fluorescence-activated Cell Sorting for Purification of Plasmacytoid Dendritic Cells from the Mouse Bone Marrow
Published on: November 4, 2016
12.6K
Conventional Type 1 Dendritic Cells (cDC1) in Human Kidney Diseases: Clinico-Pathological Correlations
Titi Chen1,2,3, Qi Cao1,2, Ruifeng Wang2
1School of Medicine, The University of Sydney, Camperdown, NSW, Australia.
Frontiers in Immunology
|May 31, 2021
Summary
Type 1 conventional dendritic cells (cDC1s) increase in various kidney diseases and correlate with disease severity and CD8+ T cell presence. This suggests cDC1s play a role in kidney pathology and immune responses.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Background:
- Conventional dendritic cell type 1 (cDC1) are crucial for cross-presentation to CD8+ T cells.
- The role and distribution of cDC1s in human kidney diseases remain incompletely understood.
Purpose of the Study:
- To investigate the quantity and location of cDC1s in diverse human kidney diseases.
- To explore the correlation between cDC1s, clinico-pathological features, and CD8+ T cells.
Main Methods:
- Analysis of 135 kidney biopsy samples representing various kidney diseases.
- Indirect immunofluorescence staining to quantify cDC1s, CD1c+ DCs, and CD8+ T cells.
Main Results:
- cDC1 numbers were elevated in acute tubular necrosis and proliferative glomerulonephritis, exceeding CD1c+ DC increases.
- cDC1s were predominantly interstitial, with notable glomerular presence in specific conditions like lupus nephritis and pauci-immune GN.
- cDC1 counts correlated with disease severity, interstitial fibrosis, crescent formation, and prognosis, and were associated with CD8+ T cell numbers and co-localization.
Conclusions:
- cDC1s are implicated in the pathogenesis of various kidney diseases, correlating with clinical and pathological outcomes.
- The association of cDC1s with CD8+ T cells suggests a coordinated immune mechanism in kidney disease progression.
Related Concept Videos
01:25Chronic Kidney Disease I: Introduction
305
Chronic Kidney Disease (CKD) arises when the kidneys progressively lose their ability to function, ultimately leading to end-stage renal disease. At this advanced stage, the kidneys can no longer filter waste or maintain essential body functions, requiring renal replacement therapy (RRT) through dialysis or a kidney transplant for survival.Early-stage chronic kidney disease and detection challengesIn CKD's early stages, symptoms often remain absent because healthy nephrons compensate for...
305
01:10Nephrons
4.3K
The kidneys are intricate organs with millions of working units known as nephrons. Each nephron features two major structures: the renal corpuscle, which facilitates blood plasma filtration, and the renal tubule, which handles the glomerular filtrate. Blood supply is directly linked to the nephrons. The renal corpuscle consists of the glomerulus, a capillary network, and the Bowman's capsule, a double-walled epithelial structure that encases the glomerulus. The filtering of blood plasma...
4.3K
01:24Chronic Kidney Disease II: Clinical Manifestations
230
Chronic Kidney Disease (CKD) progressively impairs multiple body systems due to the accumulation of uremic toxins, which disrupt cellular functions across various organs.Neurologic symptomsNeurologic symptoms often arise early in CKD, as uremic toxin buildup drives changes in cognitive and motor functions. Patients frequently experience fatigue, headache, confusion, difficulty concentrating, and, in severe cases, seizures. Peripheral neuropathy commonly manifests as burning sensations in the...
230
01:24Renal Tubule and Collecting Duct
2.1K
The renal tubule is divided into three parts: the proximal convoluted tubule (PCT), the Loop of Henle (LOH), and the distal convoluted tubule (DCT).
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
Proximal Convoluted Tubule (PCT):
The PCT is the initial segment of the renal tubule, extending from the Bowman's capsule that encloses the glomerulus. Its convoluted structure and microvilli-lined cells increase the surface area for reabsorption. The PCT reabsorbs glucose, amino acids, sodium, and water from the filtrate, ensuring essential...
2.1K
01:20Renal Corpuscle
4.7K
The glomerulus and Bowman's capsule are two essential components of the nephron, which is the functional unit of the kidney. These microscopic structures play a critical role in the process of blood filtration to produce urine.
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
Glomerulus: Structure and Function
The glomerulus is a tiny, intricate network of capillaries located at the beginning of the nephron. It's enveloped by the Bowman's capsule and receives its blood supply from an afferent arteriole, which divides into numerous...
4.7K