Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Coordination of Gene Expression Processes in Bacteria01:29

Coordination of Gene Expression Processes in Bacteria

287
The DNA replication, transcription, and translation processes are intricately coupled in bacteria, allowing efficient gene expression and rapid protein synthesis. While this physical and functional coordination is advantageous, it introduces challenges that bacteria overcome through specific regulatory mechanisms.Coupling of Replication, Transcription, and TranslationThe coupling of replication, transcription, and translation is a hallmark of bacterial gene expression. As the replisome unwinds...
287
Cytoskeletal Proteins in Bacteria01:29

Cytoskeletal Proteins in Bacteria

3.8K
Bacterial cells were initially considered simple, randomly organized structures lacking a cytoskeleton. However, the discovery of cytoskeleton homologs in bacteria led to the change of this opinion. Bacterial cytoskeletal filaments regulate the cell shape, cell polarity, cell division, and partitioning of plasmids during cell division. It was later discovered that bacterial cytoskeletal proteins, mainly actin and tubulin homologs, are diverse compared to their eukaryotic counterparts. On the...
3.8K
Biosynthesis in Bacteria01:24

Biosynthesis in Bacteria

294
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
294
Eukaryotic Compartmentalization01:46

Eukaryotic Compartmentalization

168.3K
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
168.3K
Methods for Controlling Microbial Growth01:29

Methods for Controlling Microbial Growth

1.1K
Microbial growth control refers to various methods employed to inhibit, reduce, or eliminate microorganisms to ensure safety and hygiene across different settings. These methods are categorized based on the target environment and the level of microbial control required.Biocides are versatile agents designed to control microorganisms by either inhibiting their growth or outright killing them. These agents work through various physical, chemical, mechanical, or biological mechanisms. The...
1.1K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

523
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
523

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Engineering the Self-Assembly of Bacterial Microcompartment Shell Proteins via Charged Mutations.

ACS nano·2026
Same author

Solvent-Dependent Mechanical Response of De Novo Helix Repeat Proteins.

The journal of physical chemistry. B·2026
Same author

Setting Boundaries: Surface Engineering of Viral-Inspired Materials.

Annual review of virology·2026
Same author

Mechanophore cross-linking enhances ballistic energy dissipation of polymers.

Nature·2026
Same author

Role of Polymer-Protein Interactions in the Dynamics of Polymer-Integrated Protein Crystals.

Journal of the American Chemical Society·2026
Same author

Screening metatranscriptomes for ultrastable RNA secondary structures reveals hidden bacteriophages and novel capsid nanomaterials.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Nov 4, 2025

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
09:24

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

9.2K

Computational and Experimental Approaches to Controlling Bacterial Microcompartment Assembly.

Yaohua Li1,2, Nolan W Kennedy3,4, Siyu Li1

  • 1Department of Material Science and Engineering, Northwestern University, Evanston, Illinois 60208, United States.

ACS Central Science
|May 31, 2021
PubMed
Summary

Researchers discovered how to control bacterial microcompartment assembly by altering protein interactions. This enables the design of custom nanoreactors for biochemical and energy applications.

More Related Videos

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.5K
Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.3K

Related Experiment Videos

Last Updated: Nov 4, 2025

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform
09:24

Assembly and Tracking of Microbial Community Development within a Microwell Array Platform

Published on: June 6, 2017

9.2K
Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly
10:17

Patterning of Microorganisms and Microparticles through Sequential Capillarity-assisted Assembly

Published on: November 4, 2021

3.5K
Single-cell Microfluidic Analysis of Bacillus subtilis
10:37

Single-cell Microfluidic Analysis of Bacillus subtilis

Published on: January 26, 2018

12.3K

Area of Science:

  • Biochemistry
  • Structural Biology
  • Synthetic Biology

Background:

  • Bacterial microcompartments (BMCs) are protein organelles that enhance metabolic efficiency and survival.
  • Metabolic engineers aim to repurpose BMCs for novel applications, requiring control over their assembly.

Purpose of the Study:

  • To elucidate the mechanisms governing the self-assembly of BMCs.
  • To provide design principles for engineering BMCs with specific morphologies for nanoreactor applications.

Main Methods:

  • Multiscale modeling and theoretical analysis.
  • Site-directed mutagenesis studies on BMC proteins.
  • Thermodynamic modeling.

Main Results:

  • Identified key protein interactions and structural parameters (preferred angles, interaction strengths) controlling BMC assembly.
  • Demonstrated that amino acid mutations can precisely tune these parameters.
  • Developed a thermodynamic model predicting BMC morphology based on protein interactions.

Conclusions:

  • Controlled protein assembly is achievable by modifying protein-protein interactions.
  • These findings offer a framework for designing BMC-based nanoreactors for biotechnology and energy.
  • Understanding BMC assembly mechanisms is crucial for harnessing their potential in synthetic biology.