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Nonsterile l-Lysine Fermentation Using Engineered Phosphite-Grown Corynebacterium glutamicum
Ming Lei1,2, Xiwei Peng1,2, Wenjun Sun1,2
1National Engineering Research Center for Biotechnology, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
ACS Omega
|May 31, 2021
Summary
Engineered Corynebacterium glutamicum to utilize phosphite, enabling robust, non-sterile l-lysine production. This phosphite utilization strategy enhances microbial contamination resistance and maintains production efficiency in industrial fermentation.
Area of Science:
- Biotechnology
- Microbial Engineering
- Industrial Microbiology
Background:
- Industrial amino acid production relies on Corynebacterium glutamicum fermentation.
- Conventional C. glutamicum processes are vulnerable to contamination, necessitating sterilization or antibiotics.
- Developing contamination-resistant fermentation is crucial for cost-efficiency.
Purpose of the Study:
- To engineer C. glutamicum for enhanced resistance to microbial contamination.
- To establish a robust fermentation process for l-lysine production under non-sterile conditions.
- To evaluate the efficacy of phosphite utilization as an anti-contamination strategy in C. glutamicum.
Main Methods:
- Optimized phosphite (Pt) dehydrogenase expression in the exeR locus of l-lysine-producing C. glutamicum.
- Utilized phosphite as a phosphorus source to confer a competitive advantage against contaminating microbes.
- Conducted batch fermentations under both sterile and non-sterile conditions to compare engineered and original strains.
Main Results:
- The engineered C. glutamicum strain efficiently utilized phosphite, providing resistance to contamination.
- Achieved 41.00 g/L l-lysine production in 60 h under non-sterile conditions.
- The original strain required 72 h for comparable l-lysine yield (40.78 g/L) under sterile conditions.
Conclusions:
- Engineered C. glutamicum demonstrates efficient l-lysine production under non-sterile conditions via phosphite utilization.
- This phosphite-based anti-contamination strategy is effective and maintains production efficiency.
- Findings support the development of more cost-effective and robust industrial amino acid fermentation processes.
