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Updated: Nov 4, 2025
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
Published on: January 12, 2015
Effects of extended pharmacological disruption of zebrafish embryonic heart biomechanical environment on cardiac
Yoke Yin Foo1, Efthymios Motakis2, Zenia Tiang3
1Department of Biomedical Engineering, National University of Singapore, Singapore, Singapore.
Background:
Biomechanical stimuli are known to be important to cardiac development, but the mechanisms are not fully understood. Here, we pharmacologically disrupted the biomechanical environment of wild-type zebrafish embryonic hearts for an extended duration and investigated the consequent effects on cardiac function, morphological development, and gene expression.
Results:
Myocardial contractility was significantly diminished or abolished in zebrafish embryonic hearts treated for 72 hours from 2 dpf with 2,3-butanedione monoxime (BDM). Image-based flow simulations showed that flow wall shear stresses were abolished or significantly reduced with high oscillatory shear indices. At 5 dpf, after removal of BDM, treated embryonic hearts were maldeveloped, having disrupted cardiac looping, smaller ventricles, and poor cardiac function (lower ejected flow, bulboventricular regurgitation, lower contractility, and slower heart rate). RNA sequencing of cardiomyocytes of treated hearts revealed 922 significantly up-regulated genes and 1,698 significantly down-regulated genes. RNA analysis and subsequent qPCR and histology validation suggested that biomechanical disruption led to an up-regulation of inflammatory and apoptotic genes and down-regulation of ECM remodeling and ECM-receptor interaction genes. Biomechanics disruption also prevented the formation of ventricular trabeculation along with notch1 and erbb4a down-regulation.
Conclusions:
Extended disruption of biomechanical stimuli caused maldevelopment, and potential genes responsible for this are identified.
