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Related Experiment Video

Updated: Nov 4, 2025

Real-time Imaging of Heterotypic Platelet-neutrophil Interactions on the Activated Endothelium During Vascular Inflammation and Thrombus Formation in Live Mice
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In Vivo Platelet Detection Using a Glycoprotein IIb/IIIa-Targeted Near-Infrared Fluorescence Imaging Probe.

Khanh Ha1,2, Xiaoxin Zheng3, Chase W Kessinger1,3

  • 1Masonic Medical Research Institute, Utica, New York 13501, United States.

ACS Sensors
|May 31, 2021
PubMed
Summary

Researchers developed a novel near-infrared fluorescence probe for activated platelets. This imaging agent, based on tirofiban, allows for rapid in vivo visualization of platelet activity in diseases like thrombosis.

Keywords:
glycoprotein IIb/IIIain vivo imagingnear-infrared fluorescenceplateletsthrombosis

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Area of Science:

  • Biomedical Imaging
  • Molecular Probes
  • Translational Medicine

Background:

  • Platelets are crucial in thrombosis, atherosclerosis, and cancer.
  • Current in vivo studies of platelet activity require more translatable imaging agents.
  • Glycoprotein IIb/IIIa inhibitors are clinically relevant for platelet targeting.

Purpose of the Study:

  • To develop a novel, translatable platelet-specific molecular imaging agent.
  • To enable rapid in vivo visualization of activated platelets.
  • To advance the clinical focus of platelet activity studies.

Main Methods:

  • Development of a near-infrared fluorescence probe utilizing tirofiban, a glycoprotein IIb/IIIa inhibitor.
  • In vitro experiments using human platelets to assess probe avidity.
  • In vivo studies in a murine deep-vein thrombosis model to evaluate imaging capabilities.

Main Results:

  • The developed probe demonstrated high avidity for activated platelets.
  • The probe exhibited a short blood half-life, facilitating rapid imaging.
  • Successful in vivo visualization of platelets within the vasculature was achieved.

Conclusions:

  • The tirofiban-based near-infrared fluorescence probe is a promising tool for activated platelet imaging.
  • This agent enables rapid in vivo visualization, supporting clinical translation.
  • The probe's characteristics are suitable for studying platelet roles in vascular diseases.