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Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

106.7K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
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Spermatogenesis01:22

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Spermatogenesis is a complex process that involves the development of sperm cells from undifferentiated stem cells in the seminiferous tubules of the testes. The process is essential for the production of mature and functional sperm cells that are capable of fertilizing an egg.
The process of spermatogenesis can be divided into mitosis, meiosis, and spermiogenesis. During mitosis, the spermatogonia or stem cells divide to produce two identical daughter cells, type A and B spermatogonia. Type-A...
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Testes: Histology01:27

Testes: Histology

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A tough, fibrous membrane, the tunica albuginea, covers the testes, extending inward to form fibrous partitions or septa, dividing them into internal compartments called lobules. Each lobule has 1 to 3 tightly coiled seminiferous tubules where sperm production occurs. These tubules merge into a tubular network at the back of the testis, known as the rete testis. It connects to 15 to 20 efferent ductules, leading to the epididymis.
The spermatogenic cells, responsible for producing sperm, are...
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Germ Cell Transplantation and Testis Tissue Xenografting in Mice
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Sertoli cell replacement in explanted mouse testis tissue supporting host spermatogenesis†.

Kazusa Higuchi1, Takafumi Matsumura1,2, Haruhiko Akiyama3

  • 1Laboratory of Biopharmaceutical and Regenerative Sciences, Institute of Molecular Medicine and Life Science, Yokohama City University Association of Medical Science.

Biology of Reproduction
|May 31, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel in vitro method to replace Sertoli cells in testes, enabling the study of spermatogenesis and potential infertility treatments. This technique successfully restored sperm production using transplanted cells without immunosuppression.

Keywords:
Sertoli cellorgan culturespermatogenesistoxin receptor-mediated cell knockout

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Area of Science:

  • Reproductive Biology
  • Cell Biology
  • Developmental Biology

Background:

  • Spermatogenesis, the process of sperm production, occurs in seminiferous tubules and relies heavily on Sertoli cells.
  • Understanding Sertoli cell function is crucial for addressing male infertility and reproductive health issues.
  • Current methods for studying Sertoli cell function in vivo are limited.

Purpose of the Study:

  • To develop and validate an in vitro Sertoli cell replacement method.
  • To establish a functional assay system for evaluating potential Sertoli cell substitutes.
  • To investigate the interactions between Sertoli cells and germ cells during spermatogenesis.

Main Methods:

  • Utilized Amh-diphtheria toxin receptor transgenic mice for targeted Sertoli cell ablation.
  • Combined organ culture with a toxin receptor-mediated cell knockout system.
  • Transplanted donor testis cells into immature testes and cultured with diphtheria toxin to replace endogenous Sertoli cells.

Main Results:

  • Successfully replaced endogenous Sertoli cells with transplanted Sertoli cells (mouse and rat) in vitro.
  • Restored spermatogenesis originating from resident germ cells after Sertoli cell replacement.
  • Demonstrated successful xenogeneic Sertoli cell transplantation (rat to mouse) without immunosuppression.

Conclusions:

  • The developed in vitro method enables functional assessment of Sertoli cell replacement and spermatogenesis.
  • This technique provides a new platform for studying spermatogenesis and its disorders.
  • The method facilitates closer investigation of Sertoli-germ cell interactions.