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Updated: Nov 4, 2025

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
Published on: January 6, 2018
Culture, Expansion and Differentiation of Human Bone Marrow Stromal Cells
Valeria Bisio1, Marion Espéli2, Karl Balabanian2
1Université de Paris, Institut de Recherche Saint-Louis, EMiLy, INSERM U1160, Paris, France. valeria.bisio@inserm.fr.
Abstract:
Mesenchymal stromal cells (MSC) are a rare, heterogeneous and multipotent population that can be isolated from several tissues. MSC were originally discovered in the bone marrow and studied for their capacity to maintain hematopoietic cells. We will describe here methods to isolate, culture, and bank MSC from human bone marrow. Then, characterization protocols by flow cytometry, clonogenic assays and doubling time evaluation will be developed. Finally, in vitro MSC culture and differentiation into osteoblasts, adipocytes, and chondrocytes will be explained. Thus, this chapter will detail all bases to work on MSC with consensus and clear methods and protocols.
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