Culture, Expansion and Differentiation of Mouse Bone-Derived Mesenchymal Stromal Cells

Mesenchymal stem/stromal cells (MSCs) are multipotent adult cells that are present in several tissues including the bone marrow (BM), in which they can differentiate in a variety of cell types such as osteoblasts, chondrocytes and adipocytes. The isolation of MSCs has been carried out by many studies that aim to control their differentiation into cartilaginous and bone cells in vitro in order to use this technology in the repair of damaged tissues. Here we describe the minimum requirements and an efficient method for isolation, expansion of mouse bone-derived multipotent mesenchymal stromal cells and their differentiation into osteoblasts, responsible for the bone matrix synthesis and mineralization.