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RNA-Seq Analysis of Differential Gene Expression in Electroporated Chick Embryonic Spinal Cord
Published on: November 1, 2014
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Systematic screening of long intergenic noncoding RNAs expressed during chicken embryogenesis
Junxiao Ren1, Quanlin Li1, Qinghe Zhang1
1National Engineering Laboratory for Animal Breeding and MOA Key Laboratory of Animal Genetics and Breeding, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Poultry Science
|May 31, 2021
Summary
This study systematically identified thousands of long noncoding RNAs (lncRNAs) during early chicken embryogenesis. Novel lincRNAs were discovered, offering insights into developmental gene regulation and functions like hematopoiesis.
Area of Science:
- Developmental Biology
- Genomics
- Molecular Biology
Background:
- Long noncoding RNAs (lncRNAs) are crucial regulators of biological processes, including embryonic development.
- Understanding lncRNA roles in avian embryogenesis is essential for deciphering developmental mechanisms.
Purpose of the Study:
- To systematically identify and characterize long noncoding RNAs (lncRNAs) during early chicken embryogenesis (days 1-8).
- To explore the characteristics and potential functions of identified lncRNAs, particularly intergenic lncRNAs (lincRNAs).
Main Methods:
- Iso-Seq and RNA-Seq were employed for comprehensive lncRNA identification.
- Weighted gene co-expression network analysis was used to associate lincRNAs with specific developmental stages and functions.
Main Results:
- Identification of 45,410 distinct lncRNAs, including 13,141 novel intergenic lncRNAs (lincRNAs).
- Chicken lincRNAs exhibit characteristics similar to mammalian lincRNAs, such as shorter lengths and stage-specific expression.
- A specific lincRNA module was associated with day 2 embryos, linked to hematopoiesis and lipid metabolism.
Conclusions:
- This study provides a comprehensive resource of lncRNAs during chicken embryogenesis.
- Identified lincRNAs offer valuable targets for future functional studies in avian development and related biological processes.
