One-step self-assembly of magnetic supramolecular metal-organic coordination functionalized MoS₂ complex as...

In the present study, a kind of magnetic supramolecular metal-organic coordination complex (SMOCC) functionalized MoS₂ was prepared with one-step in aqueous solution for enzyme immobilization. As possessing a protective nanocoating of PDA/PEI/Cu²⁺ (polydopamine: PDA, polyethyleneimine: PEI), the proposed material can provide biocompatible microenvironment and flexible adhesion force on particle interface, which is conductive to loading laccase (170.0 ± 1.8 mg/g) with high activity (93.0 ± 1.1 %). Compared with the free laccase, the immobilized laccase has higher stability in a broader range of pH (3-10), temperature (20-80 °C), storage time (1-18 days) and reusability (1-16 cycles). The removal of carcinogenic persistent organic pollutant malachite green in the water with the immobilized laccase shows a higher efficiency (89.4 ± 1.2 %) than free laccase (16.2 ± 0.2 %). The Fe₃O₄@MoS₂@(PDA/PEI/Cu²⁺) nanocomposites can also be used successfully to immobilize trypsin, lipase and catalase respectively, showing a satisfactory enzyme loading (157.0 ± 0.1 mg/g, 151.6 ± 1.4 mg/g, 162.6 ± 1.6 mg/g, respectively) and activity (95.0 ± 0.5 %, 90.0 ± 0.8 %, 91.0 ± 0.9 %, respectively). The MoS₂ can be replaced by carbon material and similar results can be obtained.