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Image-guided, Laser-based Fabrication of Vascular-derived Microfluidic Networks
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Self-Standing Photo-Crosslinked Hydrogel Construct: in vitro Microphysiological Vascular Model.

Amrutha Manigandan1, Preethy Amruthavarshini R1, Swaminathan Sethuraman1

  • 1Tissue Engineering & Additive Manufacturing Lab, Center for Nanotechnology & Advanced Biomaterials, SASTRA Deemed-to-be University, Thanjavur, Tamil Nadu, India.

Cells, Tissues, Organs
|May 31, 2021
PubMed
Summary

Researchers developed a stable, self-standing hydrogel construct for vascular microphysiological systems (MPS). This 3D model mimics native vasculature, enabling precise drug response and toxicity prediction in vitro.

Keywords:
AlginateBiomaterialBiomedical engineeringVessels

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Area of Science:

  • Biomedical Engineering
  • Vascular Biology
  • Drug Discovery

Background:

  • Developing physiologically equivalent vascular microphysiological systems (MPS) for drug screening is challenging due to difficulties in replicating the native vascular microenvironment.
  • Existing methods like microfluidics and 3D biofabrication have limitations in achieving hierarchical cellular arrangement, scalability, and structural integrity.

Purpose of the Study:

  • To develop a stable, viable, self-standing, and perfusable hydrogel construct for advanced vascular MPS applications.
  • To overcome the limitations of current vascular modeling techniques by creating a facile and mechanically stable construct.

Main Methods:

  • Fabrication of a dual-crosslinked hydrogel tubular construct using a rapid and scalable strategy.
  • Encapsulation of multilayered human vascular cells, including smooth muscle cells, within the hydrogel matrix.
  • Assessment of structural stability, end-to-end perfusability, and nonhemolytic behavior with red blood cells.

Main Results:

  • The fabricated tubular constructs demonstrated structural stability and end-to-end perfusability, functioning as self-standing perfusable structures.
  • The hydrogel construct exhibited nonhemolytic behavior, allowing for the perfusion of red blood cells within the luminal channel.
  • A homogenous distribution of viable smooth muscle cells was achieved throughout the dual-crosslinked hydrogel construct.

Conclusions:

  • The developed dual-crosslinked hydrogel construct is stable, viable, self-standing, and perfusable, making it a promising platform for vascular MPS.
  • This novel construct effectively recapitulates key features of native vasculature, offering improved potential for accurate drug response and toxicity prediction.
  • The facile and scalable fabrication strategy addresses limitations in current vascular modeling, advancing the field of in vitro drug screening.