Assessment of Mitochondrial Ca²⁺ Uptake

Mitochondrial Ca²⁺ uptake regulates mitochondrial function and contributes to cell signaling. Accordingly, quantifying mitochondrial Ca²⁺ signals and elaborating the mechanisms that accomplish mitochondrial Ca²⁺ uptake are essential to gain our understanding of cell biology. Here, we describe the benefits and drawbacks of various established old and new techniques to assess dynamic changes of mitochondrial Ca²⁺ concentration ([Ca²⁺]_mito) in a wide range of applications.