Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure and function of vanadium-containing bromoperoxidases.

R Wever1, B E Krenn, E De Boer

  • 1Laboratory of Biochemistry, University of Amsterdam, The Netherlands.

Progress in Clinical and Biological Research
|January 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Short-wave world revisited: Resonance in a two-dimensional cochlear model.

Hearing research·2026
Same author

Lessons learned during implementation of MR-guided High-Intensity Focused Ultrasound treatment of uterine fibroids.

Insights into imaging·2021
Same author

Avoiding Unnecessary Major Rectal Cancer Surgery by Implementing Structural Restaging and a Watch-and-Wait Strategy After Neoadjuvant Radiochemotherapy.

Annals of surgical oncology·2020
Same author

Optimization of pulmonary emphysema quantification on CT scans of COPD patients using hybrid iterative and post processing techniques: correlation with pulmonary function tests.

Insights into imaging·2019
Same author

Microbiological Quality of Commercial Tempeh in The Netherlands.

Journal of food protection·2019
Same author

Survival of Campylobacter jejuni during Poultry Processing and Pig Slaughtering.

Journal of food protection·2019
Same journal

DNA homology and chromosome stability: a sensitive yeast genetic system for identifying double-stranded DNA damage.

Progress in clinical and biological research·2018
Same journal

Endotoxin and sepsis: molecular mechanisms of pathogenesis, host resistance, and therapy. Proceedings of the 4th Conference of the International Endotoxin Society. Nagoya, Japan, October 23-27, 1996.

Progress in clinical and biological research·1998
Same journal

The pathogenic role of LBP in gram-negative sepsis and septic shock.

Progress in clinical and biological research·1998
Same journal

The role of interleukin 6 in endotoxin-induced inflammatory responses.

Progress in clinical and biological research·1998
Same journal

Role of nitric oxide and reactive oxygen species in endotoxin shock.

Progress in clinical and biological research·1998
Same journal

Interaction of lipopolysaccharide with a mammalian lyso-phosphatidate acyltransferase (LPAAT) transfected into E. coli, and effect of lisofylline on LPAAT transfected into mammalian cells.

Progress in clinical and biological research·1998
See all related articles

Vanadium bromoperoxidases from marine organisms contain vanadyl cations (VO2+) upon reduction, with similar active site structures primarily using oxygen donors. These enzymes catalyze bromination, with vanadate (VO43-) incorporation and a proposed peroxo-vanadium intermediate.

Area of Science:

  • Biochemistry
  • Bioinorganic Chemistry
  • Enzymology

Background:

  • Vanadium-containing bromoperoxidases are enzymes found in marine algae and lichens.
  • These enzymes play a role in halogenation reactions, specifically bromination.

Purpose of the Study:

  • To investigate the properties and active site structure of vanadium bromoperoxidases from Ascophyllum nodosum, Laminaria saccharina, and Xanthoria parietina.
  • To elucidate the mechanism of action and the role of vanadium in the catalytic process.

Main Methods:

  • Electron Paramagnetic Resonance (EPR) spectroscopy was used to study the vanadium ion's oxidation state and coordination environment.
  • Enzyme activity assays were performed to assess brominating activity, substrate kinetics, and inhibition patterns.
  • Amino acid composition analysis was conducted.

Related Experiment Videos

Main Results:

  • EPR spectra revealed a vanadyl cation (VO2+) upon reduction, indicating a vanadium(IV) state, while native enzymes contain vanadium(V).
  • The active site structure is similar across the studied enzymes, with oxygen donors coordinating the vanadium ion.
  • Enzymatic activity was lost upon dialysis but could be reconstituted by adding vanadate (VO43-), suggesting vanadate incorporation.
  • A catalytic mechanism involving a peroxo-vanadium(V) intermediate and subsequent oxidation of bromide to hypobromite was proposed.
  • Enzymes exhibited distinct pH optima, specific activities, and Km values for bromide, with the lichen enzyme showing nitrate inhibition.

Conclusions:

  • Vanadium bromoperoxidases incorporate vanadate and utilize a peroxo-vanadium intermediate for bromination.
  • Despite similar active site structures, the enzymes display varied catalytic properties and sensitivities.
  • The findings contribute to understanding the bioinorganic chemistry and catalytic mechanisms of vanadium-dependent enzymes.