Related Experiment Video
Updated: Nov 3, 2025
Genome-Wide Analysis of DNA Methylation in Gastrointestinal Cancer
Published on: September 18, 2020
Transcriptome Analysis and the Prognostic Role of NUDC in Diffuse and Intestinal Gastric Cancer
Sang-Ho Jeong1,2, Miyeong Park3, Sun Yi Park1
1Department of Surgery, School of Medicine, 26720Gyeongsang National University, Jinju, South Korea.
This study compared gene expression in signet ring cell gastric cancer and well-differentiated gastric cancer, identifying NUDC as a potential prognostic marker. NUDC-positive patients showed significantly better survival rates, suggesting its role in gastric cancer prognosis.
Area of Science:
- Oncology
- Genomics
- Molecular Biology
Background:
- Gastric cancer subtypes, diffuse-type and intestinal-type, exhibit distinct prognoses.
- Signet ring cell carcinoma (a diffuse-type) and well-differentiated adenocarcinoma (an intestinal-type) represent extremes in gastric cancer prognosis.
- Limited research exists on the transcriptomic differences between these gastric cancer subtypes.
Purpose of the Study:
- To compare the transcriptomes of signet ring cell gastric cancer and well-differentiated gastric cancer.
- To identify novel genes associated with gastric cancer subtypes and prognosis.
- To investigate the prognostic significance of Nuclear Distribution Protein C (NUDC) in gastric cancer.
Main Methods:
- Next-generation sequencing was performed on surgical samples from 5 well-differentiated gastric cancers and 3 signet ring cell gastric cancers.
- Gene enrichment and functional annotation analysis were conducted using the Database for Annotation, Visualization and Integrated Discovery (DAVID) bioinformatics resources.
- Immunohistochemistry was employed to validate NUDC expression in gastric cancer tissues.
Main Results:
- Significant differences in gene expression were observed between the two subtypes, with 900 genes upregulated and 644 downregulated in signet ring cell gastric cancer compared to well-differentiated gastric cancer.
- Variant analysis identified 52 variants and 30 cancer driver gene candidates, including NUDC.
- Cox proportional hazard analysis revealed T stage, N stage, and NUDC expression as independent prognostic factors for survival (P < 0.05).
- Kaplan-Meier survival analysis demonstrated significantly higher overall survival in the NUDC-positive group (53.2 ± 0.92 months) compared to the NUDC-negative group (44.6 ± 3.7 months) (P = 0.001).
Conclusions:
- The study identified 30 cancer driver gene candidates differentiating gastric cancer subtypes.
- NUDC expression was found to be an independent prognostic factor in gastric cancer.
- NUDC-positive patients exhibited significantly better survival outcomes, highlighting NUDC's potential as a prognostic biomarker.
More Related Videos
10:28Gene Regulation and Targeted Therapy in Gastric Cancer Peritoneal Metastasis: Radiological Findings from Dual Energy CT and PET/CT
Published on: January 22, 2018
09:16Detection of a CDH1 Rare Transcript Variant in Fresh-frozen Gastric Cancer Tissues by Chip-based Digital PCR
Published on: February 5, 2018
Related Concept Videos
02:55The Nucleolus
01:05Cancer-Critical Genes II: Tumor Suppressor Genes
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...