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Circular RNA 0102049 suppresses the progression of osteosarcoma through modulating miR-520g-3p/PLK2 axis

Xianliao Zhang1, Zhengbo Hu2, Wenhu Li2

  • 1Department of Orthopedics, Zhujiang Hospital of Southern Medical University, Guangzhou, China.

Bioengineered
|June 1, 2021
PubMed

Insights

Circular RNAs (circRNAs) play a role in osteosarcoma (OS) progression. This study shows circ_0102049 suppresses OS by regulating the miR-520g-3p/PLK2 axis, offering a potential therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Circular RNAs (circRNAs) are implicated in human cancer, including osteosarcoma (OS).
  • The specific role of circ_0102049 in OS progression is currently unknown.
  • circ_0102049 is highly expressed in OS cell lines.

Purpose of the Study:

  • To elucidate the function and mechanism of circ_0102049 in osteosarcoma.
  • To investigate the regulatory pathway involving circ_0102049, microRNA-520g-3p (miR-520g-3p), and Polo-like kinase 2 (PLK2) in OS.

Main Methods:

  • In vivo and in vitro experiments using osteosarcoma cell lines (MG63).
  • RNA interference techniques to silence circ_0102049.
  • Luciferase reporter assays to validate the interaction between miR-520g-3p and PLK2.
  • Analysis of proliferation, invasion, migration, cell cycle, and apoptosis.

Main Results:

  • Silencing circ_0102049 exacerbated OS tumorigenesis in vivo.
  • circ_0102049 acts as a sponge for miR-520g-3p.
  • miR-520g-3p directly targets and suppresses PLK2 expression.
  • circ_0102049 knockdown promoted proliferation, invasion, migration, and cell-cycle progression while inhibiting apoptosis in OS cells via the miR-520g-3p/PLK2 axis.

Conclusions:

  • circ_0102049 suppresses osteosarcoma progression through the miR-520g-3p/PLK2/TAp73 axis.
  • circ_0102049 represents a potential therapeutic target for osteosarcoma treatment.
Keywords:
OsteosarcomaPLK2circ_0102049miR-520g-3p

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