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Updated: Nov 3, 2025
Selective Cleaning of Wild Caenorhabditis Nematodes to Enrich for Intestinal Microbiome Bacteria
Published on: August 13, 2021
Rapid preparation of gastrointestinal nematode eggs from faeces for PCR identification
Nicole Hedley1, D Grant Richards2, Enzo A Palombo1
1Department of Chemistry and Biotechnology, Swinburne University of Technology, John Street, Hawthorn, VIC 3122, Australia.
Abstract:
Detection of gastrointestinal nematodes (GIN) as both a qualitative and quantitative test is highly desirable. Methods such as multiplex and qPCR are capable of providing such results, but can be laborious and expensive. This paper presents a rapid, low-cost method of preparing GIN egg from faecal samples that produces DNA suitable for PCR analysis. We also describe a set of primers that are suitable for single-tube multiplex PCR.
