Fibroblast functionality on novel Ti30Ta nanotube array
Patricia Capellato1, Barbara S Smith2, Ketul C Popat3
1Department of Materials, Faculty of Engineering Guaratinguetá, Sao Paulo State University-UNESP, Av. Ariberto Pereira da Cunha, 333, Pedregulho, CEP 12516-410, Guaratinguetá, SP, Brazil.
Abstract:
In this study, the mechanical substrate and topographical surface properties of anodized Ti30Ta alloy were investigated using scanning electron microscopy (SEM), energy-dispersive spectroscopy (EDS) and contact angle measurement. The anodization process was performed in an electrolyte solution containing HF (48%) and H2SO4 (98%) in the volumetric ratios 1:9 with the addition of 5% dimethyl sulfoxide (DMSO) at 15V, 25V and 35V for 20 and 40min, producing a nanotube architecture when anodized at 35V for 40min. Human dermal fibroblasts (HDF, neonatal) were utilized to evaluate the biocompatibility of Ti30Ta nanotubes and Ti30Ta alloy after 1 and 3days of culture. Cellular adhesion, proliferation, viability, cytoskeletal organization and morphology were investigated using fluorescence microscope imaging, biochemical assay and SEM imaging respectively. The results presented identify altered material properties and improved cellular interaction on Ti30Ta nanotubes as compared to Ti30 Ta alloy.
